000163849 001__ 163849
000163849 005__ 20251107115329.0
000163849 0247_ $$2doi$$a10.1016/j.mtchem.2025.103109
000163849 0248_ $$2sideral$$a145859
000163849 037__ $$aART-2025-145859
000163849 041__ $$aeng
000163849 100__ $$aDereje, Degnet Melese
000163849 245__ $$aPolymeric dye-loaded electrospun fibers for antimicrobial photodynamic therapy
000163849 260__ $$c2025
000163849 5060_ $$aAccess copy available to the general public$$fUnrestricted
000163849 5203_ $$aAs an alternative to the extensive use and misuse of antibiotics, antimicrobial photodynamic therapy stands out as a non-selective, broad-spectrum, locally and spatially controlled antimicrobial therapy. Reactive oxygen and nitrogen species, generated by appropriate light on specific photosensitizers in the presence of tissue oxygen, cause rapid bacterial damage with reduced chances for bacteria to develop adaptive mechanisms of acquired or genetic resistance. Herein, we developed a light-responsive advanced wound dressing based on electrospun polycaprolactone (PCL) nanofibers decorated with PLGA microparticles (MPs) encapsulating a polymethine cyanine dye (Br-CY5). This bilayer system was designed to enable bacterial inactivation upon light irradiation while providing sustained dye release and mechanical stability suited for chronic wound environments. The hybrid dressing was evaluated against Staphylococcus aureus, which is a bacterial pathogen commonly present on chronic infected wounds, addressing a 99.9 % reduction in the bacterial load after irradiating the dressing with a 640 nm LED for 15 min. Additionally, the cytotoxicity of the free dye and the developed dressing was assessed on various eukaryotic cell lines to ensure cytocompatibility. These results highlight the potential of this bilayered photodynamic system as an effective and safe antimicrobial wound dressing for managing chronic infections.
000163849 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2023-146091OB-I00
000163849 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000163849 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000163849 700__ $$aPontremoli, Carlotta
000163849 700__ $$0(orcid)0000-0002-1505-498X$$aYus, Cristina$$uUniversidad de Zaragoza
000163849 700__ $$0(orcid)0000-0002-0272-3152$$aGámez, Enrique
000163849 700__ $$aPaesa, Monica$$uUniversidad de Zaragoza
000163849 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia
000163849 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000163849 700__ $$aBarbero, Nadia
000163849 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000163849 773__ $$g49 (2025), 103109 [12 pp.]$$tMaterials Today Chemistry$$x2468-5194
000163849 8564_ $$s8634296$$uhttps://zaguan.unizar.es/record/163849/files/texto_completo.pdf$$yVersión publicada
000163849 8564_ $$s2637578$$uhttps://zaguan.unizar.es/record/163849/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000163849 909CO $$ooai:zaguan.unizar.es:163849$$particulos$$pdriver
000163849 951__ $$a2025-11-07-10:25:57
000163849 980__ $$aARTICLE