000163855 001__ 163855
000163855 005__ 20251107115329.0
000163855 0247_ $$2doi$$a10.1007/s11060-025-05253-0
000163855 0248_ $$2sideral$$a145938
000163855 037__ $$aART-2025-145938
000163855 041__ $$aeng
000163855 100__ $$0(orcid)0000-0003-2672-5790$$aBaselga, Marta
000163855 245__ $$aThree-dimensional ultrastructural analysis of glioblastoma reveals spheresome-mediated intercellular communication
000163855 260__ $$c2025
000163855 5203_ $$aPurpose: The tumor microenvironment plays a central role in glioblastoma progression and treatment resistance, with extracellular vesicles (EVs) mediating much of the intercellular communication. Here, we provide the first ultrastructural evidence of spheresomes -a specific EV subtype- predominance in human glioblastomas, together with novel observations on their transport and interactions.
Methods: Tumor samples from four patients with histologically confirmed glioblastoma were examined using transmission electron microscopy (TEM) and three-dimensional (3D) reconstructions from ultrathin serial sections.
Results: In glioblastomas, spheresomes, contained within multivesicular spheres (MVSs), were the most prevalent EV subtype, exceeding multivesicular bodies containing exosomes in number and size. 3D reconstructions revealed numerous contacts between glioblastoma tumor cells and MVSs, active release events, and occasional traversal of endothelial cells into the vascular lumen, suggesting potential for both local and systemic transport. Additionally, we report independent and descriptive new ultrastructural observations of glioblastoma. These tumors well-differenciated cells exhibited irregular nuclei with nuclear blebs and nuclear envelope-limited chromatin sheets, as well as cytoplasmic concentric rough endoplasmic reticulum laminations, membranous stacks, crystalline bodies, and concentric rearrangements of gliofilaments. Primary cilia (9 + 0) were also observed.
Conclusions: These findings expand the ultrastructural profile of glioblastoma, highlight spheresomes as a potentially important EV population in tumor communication, and underscore the value of further molecular studies to evaluate their role as potential biomarkers.
000163855 536__ $$9info:eu-repo/grantAgreement/ES/DGA/PROY_B21_24
000163855 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000163855 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000163855 700__ $$aBerna, Sophia
000163855 700__ $$aMedina Pérez, Víctor Manuel
000163855 700__ $$aSoriano, Mario
000163855 700__ $$aGiraldo, Cindy
000163855 700__ $$0(orcid)0000-0001-8567-4327$$aMuñoz, Guillermo$$uUniversidad de Zaragoza
000163855 700__ $$0(orcid)0000-0003-3883-3094$$aCalatayud-Pérez, Juan$$uUniversidad de Zaragoza
000163855 700__ $$0(orcid)0000-0001-5821-3920$$aAguas, Jesús$$uUniversidad de Zaragoza
000163855 700__ $$aSchuhmacher, Alberto J.
000163855 700__ $$0(orcid)0000-0002-9951-1075$$aJunquera, Concepción
000163855 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía
000163855 7102_ $$11013$$2020$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Anatomía Patológica
000163855 773__ $$g176, 1 (2025), [11 pp.]$$pJ. neuro-oncol.$$tJOURNAL OF NEURO-ONCOLOGY$$x0167-594X
000163855 8564_ $$s7940163$$uhttps://zaguan.unizar.es/record/163855/files/texto_completo.pdf$$yVersión publicada
000163855 8564_ $$s2398013$$uhttps://zaguan.unizar.es/record/163855/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000163855 909CO $$ooai:zaguan.unizar.es:163855$$particulos$$pdriver
000163855 951__ $$a2025-11-07-10:26:03
000163855 980__ $$aARTICLE