000163918 001__ 163918
000163918 005__ 20251113160752.0
000163918 0247_ $$2doi$$a10.1007/s00216-020-02698-2
000163918 0248_ $$2sideral$$a118222
000163918 037__ $$aART-2020-118222
000163918 041__ $$aeng
000163918 100__ $$0(orcid)0000-0003-2685-5739$$aNerín, C.$$uUniversidad de Zaragoza
000163918 245__ $$aInfluence of nonylphenol from multilayer plastic films on artificial insemination of sows
000163918 260__ $$c2020
000163918 5060_ $$aAccess copy available to the general public$$fUnrestricted
000163918 5203_ $$aArtificial insemination is common practice in mass livestock farming. Recently, it was shown that chemicals leaching from multilayer plastic bags affect the fertility of boars, although common quality tests did not show any impact on the sperm. It is not clear whether this incidence was a single case or whether it could be a systematic problem. Therefore, we studied six multilayer plastic bags. A total of 49 compounds were found, but most of them were at very low intensity. Nonylphenols in the range of 19-99 µg/g plastic were found. Migration tests using water and 10% ethanol as simulants, to mimic the behavior of semen with the extender, were performed. The most interesting migrants in terms of potential reprotoxicity were identified as nonylphenols. The identification in depth demonstrated the presence of 10 isomers of nonylphenol with a total concentration range between 16 to 58 µg/Kg simulant, among other migrants at lower concentration. The influence of these nonylphenols and their maximum tolerable concentration in direct contact with semen from boars was studied. Motility, viability, mitochondrial activity and acrosomes reacted were significantly affected at 10 mg/Kg of nonylphenols in contact with the sperm, but in vitro penetration rate was significantly decreased with only 2 mg/Kg. Insight into the mode of action is also provided.
000163918 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/T53-17R
000163918 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000163918 590__ $$a4.142$$b2020
000163918 591__ $$aCHEMISTRY, ANALYTICAL$$b22 / 83 = 0.265$$c2020$$dQ2$$eT1
000163918 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b20 / 77 = 0.26$$c2020$$dQ2$$eT1
000163918 592__ $$a0.86$$b2020
000163918 593__ $$aBiochemistry$$c2020$$dQ1
000163918 593__ $$aAnalytical Chemistry$$c2020$$dQ1
000163918 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000163918 700__ $$aSu, Q.Z.
000163918 700__ $$0(orcid)0000-0002-8765-4319$$aVera, P.$$uUniversidad de Zaragoza
000163918 700__ $$aMendoza, N.
000163918 700__ $$aAusejo, R.
000163918 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000163918 7102_ $$12009$$2X$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cProy. investigación HVA
000163918 773__ $$g2020, 412 (2020), 6519–6528$$pAnal. Bioanal. Chem.$$tAnalytical and Bioanalytical Chemistry$$x1618-2642
000163918 8564_ $$s743504$$uhttps://zaguan.unizar.es/record/163918/files/texto_completo.pdf$$yPostprint
000163918 8564_ $$s2481957$$uhttps://zaguan.unizar.es/record/163918/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000163918 909CO $$ooai:zaguan.unizar.es:163918$$particulos$$pdriver
000163918 951__ $$a2025-11-13-14:57:49
000163918 980__ $$aARTICLE