163918 20251113160752.0 doi 10.1007/s00216-020-02698-2 sideral 118222 ART-2020-118222 eng Nerín, C. Universidad de Zaragoza (orcid)0000-0003-2685-5739 Influence of nonylphenol from multilayer plastic films on artificial insemination of sows 2020 Artificial insemination is common practice in mass livestock farming. Recently, it was shown that chemicals leaching from multilayer plastic bags affect the fertility of boars, although common quality tests did not show any impact on the sperm. It is not clear whether this incidence was a single case or whether it could be a systematic problem. Therefore, we studied six multilayer plastic bags. A total of 49 compounds were found, but most of them were at very low intensity. Nonylphenols in the range of 19-99 µg/g plastic were found. Migration tests using water and 10% ethanol as simulants, to mimic the behavior of semen with the extender, were performed. The most interesting migrants in terms of potential reprotoxicity were identified as nonylphenols. The identification in depth demonstrated the presence of 10 isomers of nonylphenol with a total concentration range between 16 to 58 µg/Kg simulant, among other migrants at lower concentration. The influence of these nonylphenols and their maximum tolerable concentration in direct contact with semen from boars was studied. Motility, viability, mitochondrial activity and acrosomes reacted were significantly affected at 10 mg/Kg of nonylphenols in contact with the sperm, but in vitro penetration rate was significantly decreased with only 2 mg/Kg. Insight into the mode of action is also provided. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA-FEDER/T53-17R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.142 2020 CHEMISTRY, ANALYTICAL 22 / 83 = 0.265 2020 Q2 T1 BIOCHEMICAL RESEARCH METHODS 20 / 77 = 0.26 2020 Q2 T1 0.86 2020 Biochemistry 2020 Q1 Analytical Chemistry 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Su, Q.Z. Vera, P. Universidad de Zaragoza (orcid)0000-0002-8765-4319 Mendoza, N. Ausejo, R. 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 2009 X Universidad de Zaragoza Dpto. Química Analítica Proy. investigación HVA 2020, 412 (2020), 6519–6528 Anal. Bioanal. Chem. Analytical and Bioanalytical Chemistry 1618-2642 743504 http://zaguan.unizar.es/record/163918/files/texto_completo.pdf Postprint 2481957 http://zaguan.unizar.es/record/163918/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:163918 articulos driver 2025-11-13-14:57:49 ARTICLE