000163921 001__ 163921
000163921 005__ 20251113160752.0
000163921 0247_ $$2doi$$a10.1080/13880209.2025.2583836
000163921 0248_ $$2sideral$$a146126
000163921 037__ $$aART-2025-146126
000163921 041__ $$aeng
000163921 100__ $$aNúñez, Sonia
000163921 245__ $$aVasorelaxant effects of the edible flowers Tagetes erecta L. and its possible mechanism of action
000163921 260__ $$c2025
000163921 5060_ $$aAccess copy available to the general public$$fUnrestricted
000163921 5203_ $$aContext
Tagetes erecta is widely cultivated for its ornamental flowers and has traditionally been used as a diuretic and antihypertensive. However, its effects on blood pressure have not yet been studied.
Objective
To evaluate the vasorelaxant potential of ethanolic extract of T. erecta from two cultivars of edible flowers, yellow and orange.
Materials and Methods
The pharmacological effects of T. erecta extracts as vasorelaxant agents were evaluated using isolated rat aorta rings in an organ bath and by measuring the pharyngeal pumping rate in the Caenorhabditis elegans model.
Results
The extracts induced relaxation in endothelium-intact aortic rings pre-contracted with different agents. Vasorelaxant effect was attenuated by endothelial removal and by pretreatment with L-NAME or ODQ, but not by indomethacin. Statistically significant effects were observed only at low concentrations. Atropine and H-89 reduced the extract-induced response, whereas okadaic acid had no effect. In a calcium-free medium, the extracts reduced contractions induced by CaCl2 and phenylephrine. Relaxation was significantly attenuated by iberiotoxin, glibenclamide, BaCl2, and 4-aminopyridine, while apamin and TRAM-34 had mild effect. The extracts also significantly decreased the pharyngeal pumping rate in C. elegans.
Discussion and Conclusion
The extracts induced concentration-dependent vasorelaxation though both endothelium-dependent and -independent mechanisms. At low concentrations, relaxation was mediated by nitric oxide, while at higher concentrations it involved inhibition of intracellular Ca2+, opening of K+ channels, and activation of protein kinase A. In C. elegans, the extracts significantly reduced pharyngeal pumping. This study is the first to suggest that T. erecta could be beneficial in treating pathologies associated with endothelial dysfunction, such as hypertension.
000163921 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B44-20D$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2018-BIO-09$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2021-BIO-08
000163921 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttps://creativecommons.org/licenses/by-nc/4.0/deed.es
000163921 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000163921 700__ $$aLópez, Víctor
000163921 700__ $$0(orcid)0000-0002-2114-0577$$aArruebo Loshuertos, María Pilar$$uUniversidad de Zaragoza
000163921 700__ $$0(orcid)0000-0002-7412-2073$$aPlaza Carrión, Miguel Ángel$$uUniversidad de Zaragoza
000163921 700__ $$aGómez-Rincón, Carlota
000163921 700__ $$0(orcid)0000-0002-2231-7565$$aValero, Marta Sofía$$uUniversidad de Zaragoza
000163921 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000163921 773__ $$g63, 1 (2025), 778-799$$pPharm. biol.$$tPHARMACEUTICAL BIOLOGY$$x1388-0209
000163921 8564_ $$s3555175$$uhttps://zaguan.unizar.es/record/163921/files/texto_completo.pdf$$yVersión publicada
000163921 8564_ $$s1093721$$uhttps://zaguan.unizar.es/record/163921/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000163921 909CO $$ooai:zaguan.unizar.es:163921$$particulos$$pdriver
000163921 951__ $$a2025-11-13-14:57:52
000163921 980__ $$aARTICLE