164004 20251121161350.0 doi 10.1007/s00216-009-2766-0 sideral 83925 ART-2009-83925 eng Bentayeb, Karim (orcid)0000-0002-3399-9987 Adaptation of the ORAC assay to the common laboratory equipment and subsequent application to antioxidant plastic films 2009 The oxygen radical absorbance capacity (ORAC) method has been adapted to the instrumental laboratory and optimized for the determination of the antioxidant capacity of a novel active packaging. As the ORAC assay requires the monitorization of a reaction at controlled temperature by means of the fluorescence signal decrease over time, specific instrumental is usually necessary. In this work, a common liquid chromatographic device has been adapted to perform the ORAC assay, leaving it accessible to any laboratory. Using this adaptation, five different essential oils have been determined resulting in the following antioxidant order: clove (2.66 g Trolox per gram of essential oil), oregano (2.25), cinnamon (1.93), rosemary (1.66), and ginger (1.47). After incorporating the essential oils to the film, its antioxidant capacity has also been checked and related to the concentration of essential oil as well as the thickness of the active film. The results point out that for the same amount of essential oil incorporated measured as grams per square meter, thicker films have more antioxidant capacity than the thinner and more concentrated ones. Furthermore, the antioxidant capacity found in the films was always higher than expected taking into account the amount of essential oil incorporated. Some likely explanations have been proposed, leading to the improvement of the antioxidant film under development Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.48 2009 CHEMISTRY, ANALYTICAL 6 / 70 = 0.086 2009 Q1 T1 BIOCHEMICAL RESEARCH METHODS 17 / 67 = 0.254 2009 Q2 T1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Vera, Paula (orcid)0000-0002-8765-4319 Rubio, Carlos Universidad de Zaragoza (orcid)0000-0002-6532-2014 Nerín, Cristina Universidad de Zaragoza (orcid)0000-0003-2685-5739 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 394, 3 (2009), 903-910 Anal. Bioanal. Chem. Analytical and Bioanalytical Chemistry 1618-2642 494513 http://zaguan.unizar.es/record/164004/files/texto_completo.pdf Postprint 2594789 http://zaguan.unizar.es/record/164004/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:164004 articulos driver 2025-11-21-14:24:32 ARTICLE