164007 20251121161350.0 doi 10.1016/j.crmicr.2025.100485 sideral 146123 ART-2025-146123 eng Salvador, Maika Universidad de Zaragoza (orcid)0000-0002-4915-3302 Characterization of germinant receptors of P. thermoglucosidasius NCIMB 11955 2025 Germinant receptors (GRs) initiate spore germination in response to nutrient signals. While their roles are well studied in Bacillus spp., less is known in strict thermophiles such as (Para)Geobacillus spp. This study evaluated the roles of the GR operons identified in Parageobacillus thermoglucosidasius NCIMB 11955 (gerK1, gerK2, and gerX1) in germination and outgrowth. The GerK1 ortholog was essential for germination in TSB supplemented with yeast extract (TSBYE), D-fructose, and D-mannose; it cooperated with GerX1 for germination in maltose, sucrose, and raffinose; with GerK2 or GerX1 for germination in D-arabinose, D-glucose, and D-xylose; and with both for germination in lactose. GerK1 also dominated colony formation on TSA supplemented with yeast extract (TSAYE), but maximal recovery required GerX1 and GerK2, suggesting their involvement in spore outgrowth. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/AEI/TED2021-129779B-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2023-148505OB-I00 info:eu-repo/semantics/openAccess by-nc https://creativecommons.org/licenses/by-nc/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Lau, Matthew S.H. Minton, Nigel P. Condón, Santiago Universidad de Zaragoza (orcid)0000-0002-1902-0648 Gayán, Elisa 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 9 (2025), 100485 [5 pp.] Current Research in Microbial Sciences 2666-5174 694940 http://zaguan.unizar.es/record/164007/files/texto_completo.pdf Versión publicada 2694617 http://zaguan.unizar.es/record/164007/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:164007 articulos driver 2025-11-21-14:24:37 ARTICLE