164034 20251121161351.0 doi 10.1002/mnfr.70304 sideral 146285 ART-2025-146285 eng Aguirre Ramírez, Diego (orcid)0000-0002-1829-6319 Modulation of intestinal microbiota through kefir‐based beverages in a Murine model of acute pancreatitis 2025 Acute pancreatitis (AP) severely disrupts gut microbiota, leading to dysbiosis. This study investigated whether kefir derived from pasteurized whole milk (PMK) or whey (WK) could mitigate AP-induced gut microbiota alterations and promote gut health in a murine model. Male BALB/cJRj mice were assigned to CONTROL, AP-induced (PAN), PMK, WK, PMKPAN, or WKPAN groups. Fecal bacterial DNA was analyzed via 16S rRNA sequencing. AP induction caused characteristic dysbiosis, increasing Marinifilaceae, Tannerellaceae, Odoribacter, and Parabacteroides. In AP mice, PMK treatment significantly reduced Tannerellaceae and Parabacteroides, while increasing potentially anti-inflammatory Marinifilaceae and butyrate-producing Odoribacter. In healthy mice, PMK reduced pro inflammatory Desulfovibrionaceae and Bilophila, enriching short-chain fatty acid (SCFA) producers like Acetatifactor and Roseburia, with pathway analysis showing increased acetate kinase activity. WK treatment in healthy animals also promoted SCFA-producers (Acetatifactor, Odoribacter) and Muribaculaceae. In the AP model, WK upregulated bacteria involved in fatty acid biosynthesis pathways. Dairy-based kefir, particularly PMK, demonstrates a significant capacity to counteract AP-induced gut microbiota imbalances. These findings highlight kefir's nutritional relevance as a functional food with potential preventive and therapeutic applications for supporting gut health in the context of AP. info:eu-repo/grantAgreement/ES/DGA/A20-23R info:eu-repo/grantAgreement/ES/MICINN/PID2022-139104OB-I00 info:eu-repo/grantAgreement/ES/UZ/UZ2021-CIE-03 info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Garrido, María Universidad de Zaragoza Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X Grasa, Laura Universidad de Zaragoza (orcid)0000-0002-5306-9365 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos (2025), e70304 [13 pp.] MOL NUTR FOOD RES MOLECULAR NUTRITION & FOOD RESEARCH 1613-4125 2622153 http://zaguan.unizar.es/record/164034/files/texto_completo.pdf Versión publicada info:eu-repo/date/embargoEnd/2026-10-29 2257898 http://zaguan.unizar.es/record/164034/files/texto_completo.jpg?subformat=icon icon Versión publicada info:eu-repo/date/embargoEnd/2026-10-29 oai:zaguan.unizar.es:164034 articulos driver 2025-11-21-14:25:10 ARTICLE