000164047 001__ 164047
000164047 005__ 20251121161351.0
000164047 0247_ $$2doi$$a10.1111/rda.70145
000164047 0248_ $$2sideral$$a146315
000164047 037__ $$aART-2025-146315
000164047 041__ $$aeng
000164047 100__ $$0(orcid)0000-0003-0770-9250$$aAusejo-Marcos, Raquel$$uUniversidad de Zaragoza
000164047 245__ $$aAcrosomal Status and PARP-1 Nuclear Markers Could Improve Discrimination of Potential Fertility in Good‐Quality Boar Semen Doses
000164047 260__ $$c2025
000164047 5060_ $$aAccess copy available to the general public$$fUnrestricted
000164047 5203_ $$aReproductive efficiency in boars partly depends on semen quality. However, it is challenging to predict sperm fertility once acceptable quality endpoints have been met. This study aims to establish a link between different semen quality parameters and farm fertility outcomes in semen doses selected for commercial use. We analysed 105 ejaculates from 15 adult Pietrain boars, extended into 45 mL artificial insemination (AI) doses. A total of 605 sows were inseminated (40.1 ± 7.8 females/boar) and fertility, farrowing rate and prolificacy data were recorded. Sperm evaluation included sperm plasma analysis, kinematics, morphology, viability, acrosome integrity, apoptotic-like changes, mitochondrial activity and DNA damage (DNA fragmentation poly-ADP ribose polymerase 1, PARP1, together with its product PAR and its cleaved form cPARP). The sperm membrane and acrosome were evaluated using the short hypo-osmotic swelling test (sHOST) and the osmotic resistance test (ORT). Fertility and farrowing rates exceeded 94%, with an average of 20.18 ± 2.03 piglets born/litter (average born alive: 87.75% ± 5.61%). Negative correlations were found between damaged acrosomes and ultrasound fertility (ρ = −0.240, p = 0.021), farrowing rate (ρ = −0.244; p = 0.019), and total born (r = −0.304; p = 0.003). Average born alive was positively correlated with plasma seminal concentrations of protein (r = 0.273; p = 0.009), fructose (r = 0.243; p = 0.024) and cathepsine B (ρ = 0.257; p = 0.029) but negatively correlated with apoptosis and DNA damage nuclear markers cPARP (r = −0.295, p = 0.005) and PAR (r = −0.209, p = 0.049). However, regression models only showed significant results for predicting the total born from damaged acrosomes and those born alive from cPARP, although the coefficients of determination were very low. Since semen quality and fertility were good, most parameters did not affect fertility outcomes. In agreement with previous studies, acrosomal damage would be a reliable predictor of reproductive outcomes, whereas cPARP would show potential as a novel biomarker.
000164047 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000164047 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000164047 700__ $$0(orcid)0000-0001-6733-9373$$aMiguel-Jiménez, Sara
000164047 700__ $$0(orcid)0000-0001-5026-5144$$aTejedor, M. Teresa$$uUniversidad de Zaragoza
000164047 700__ $$aGómez-Giménez, Belén
000164047 700__ $$aSoriano-Úbeda, Cristina
000164047 700__ $$aMartinez-Pastor, Felipe
000164047 700__ $$aMendoza, Noelia
000164047 700__ $$aVicente-Carrillo, Alejandro
000164047 700__ $$aHurtado, William Fernando
000164047 700__ $$aÁvila Holguín, Celia
000164047 700__ $$0(orcid)0000-0001-6629-6842$$aMoreno, Bernardino$$uUniversidad de Zaragoza
000164047 700__ $$0(orcid)0000-0001-6964-7011$$aFalceto, María Victoria$$uUniversidad de Zaragoza
000164047 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000164047 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000164047 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000164047 773__ $$g60, 11 (2025), e70145 [7 pp.]$$pReprod. domest. anim.$$tReproduction in Domestic Animals$$x0936-6768
000164047 8564_ $$s260078$$uhttps://zaguan.unizar.es/record/164047/files/texto_completo.pdf$$yVersión publicada
000164047 8564_ $$s2339851$$uhttps://zaguan.unizar.es/record/164047/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000164047 909CO $$ooai:zaguan.unizar.es:164047$$particulos$$pdriver
000164047 951__ $$a2025-11-21-14:25:27
000164047 980__ $$aARTICLE