000164108 001__ 164108
000164108 005__ 20251121161351.0
000164108 0247_ $$2doi$$a10.1021/acs.analchem.5c04341
000164108 0248_ $$2sideral$$a146255
000164108 037__ $$aART-2025-146255
000164108 041__ $$aeng
000164108 100__ $$0(orcid)0000-0001-9582-6283$$aRua-Ibarz, Ana$$uUniversidad de Zaragoza
000164108 245__ $$aDiscrete Entity Analysis via Microwave-Induced Nitrogen Plasma–Mass Spectrometry in Single-Event Mode
000164108 260__ $$c2025
000164108 5060_ $$aAccess copy available to the general public$$fUnrestricted
000164108 5203_ $$aIn this work, single-event microwave-induced nitrogen plasma–mass spectrometry (single-event MINP-MS) was evaluated for the first time for the analysis of discrete entities such as nanoparticles, biological cells, and microplastics. Nitrogen (N2) effectively overcomes Ar-based polyatomic interferences, enabling (ultra)trace element determination of Fe and Se using their most abundant isotopes, 56Fe (91.66%) and 80Se (49.82%). Iron oxide nanoparticles (Fe2O3 NPs) ranging from 20 to 70 nm were accurately characterized, with excellent agreement with established sizing techniques, such as transmission electron microscopy (TEM) and dynamic light scattering (DLS). A limit of detection (LoD) of 8.6 ag for Fe─equivalent to an LoDsize of 19 nm for Fe2O3─was achieved, which is significantly lower than recent values reported for high-end quadrupole-based ICP-MS. Selenium nanoparticles (SeNPs) of 150 and 250 nm were also accurately characterized, without the N2-based plasma experiencing issues handling relatively large metallic NPs (linearity, R2 = 0.9994). Se-enriched yeast cells (SELM-1 certified reference material) were successfully analyzed via single-cell MINP-MS using external calibration based on SeNPs and a transport efficiency-independent approach. In addition, 2–3 μm polystyrene (PS) and polytetrafluoroethylene (PTFE) were accurately sized by monitoring 12C+, confirming the method’s suitability for handling micrometer-sized polymeric materials (microplastics). The average duration of individual events (680 ± 160 μs) suggests that the digestion of individual entities in N2-based plasmas is comparable to that in Ar-based plasmas. These results open new avenues for this instrumentation as an alternative to ICP ionization sources, also in the context of discrete entity analysis.
000164108 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E43-20R$$9info:eu-repo/grantAgreement/ES/DGA/PROY_E17_24$$9info:eu-repo/grantAgreement/EC/H2020/101034288/EU/International Fellowship Programme for Talent Attraction to the Campus of International Excellence Campus Iberus/IberusExperience$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101034288-IberusExperience$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-122455NB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2021-031093-I
000164108 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000164108 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000164108 700__ $$0(orcid)0000-0001-7087-9901$$aNakadi, Flávio V.$$uUniversidad de Zaragoza
000164108 700__ $$0(orcid)0000-0002-1856-2058$$aBolea-Fernandez, Eduardo$$uUniversidad de Zaragoza
000164108 700__ $$aBazo, Antonio$$uUniversidad de Zaragoza
000164108 700__ $$aBattistella, Beatrice
000164108 700__ $$aMatiushkina, Anna
000164108 700__ $$aResch-Genger, Ute
000164108 700__ $$aAbad, Carlos
000164108 700__ $$0(orcid)0000-0002-7450-8769$$aResano, Martín$$uUniversidad de Zaragoza
000164108 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000164108 773__ $$g97, 43 (2025), 24065-24072$$pAnal. chem.$$tANALYTICAL CHEMISTRY$$x0003-2700
000164108 8564_ $$s3931154$$uhttps://zaguan.unizar.es/record/164108/files/texto_completo.pdf$$yVersión publicada
000164108 8564_ $$s3061709$$uhttps://zaguan.unizar.es/record/164108/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000164108 909CO $$ooai:zaguan.unizar.es:164108$$particulos$$pdriver
000164108 951__ $$a2025-11-21-14:26:40
000164108 980__ $$aARTICLE