000164122 001__ 164122
000164122 005__ 20251121161351.0
000164122 0247_ $$2doi$$a10.1111/jpn.70024
000164122 0248_ $$2sideral$$a146273
000164122 037__ $$aART-2025-146273
000164122 041__ $$aeng
000164122 100__ $$0(orcid)0000-0001-5880-6021$$aBelanche, Alejandro$$uUniversidad de Zaragoza
000164122 245__ $$aIncreasing rumen microbial diversity in goats favours the adaptation to high‐concentrate diets with minor effects on feed utilization
000164122 260__ $$c2025
000164122 5060_ $$aAccess copy available to the general public$$fUnrestricted
000164122 5203_ $$aEvolution has enabled ruminants to develop a complex rumen microbiota that aids in the digestion of fibrous feeds. This study examines whether promoting a highly diverse rumen microbiota during early life continues to offer long‐term benefits in modern dairy production systems, where young ruminants are reared without exposure to adult ruminants and are fed high‐concentrate diets. A total of 36 newborn goat kids were divided in 4 groups. During the first 10 weeks of age, animals were daily inoculated with autoclaved rumen fluid (AUT), fresh rumen fluid from adult goats fed forage (RFF), or concentrate diet (RFC), or received no inoculation (CTL). At 29 weeks of age, following an 18‐week wash out period, the animals were shifted from a full‐forage to a high‐concentrate diet to assess their ability to adapt and digest this later diet. Results revealed that early life inoculation with fresh rumen fluid had a lasting effect on the rumen microbiota, promoting higher bacterial (+93 OTUs), methanogens (+5 OTUs) and protozoal diversity (+23 OTUs), whereas CTL animals remained protozoa‐free. This superior microbial complexity accelerated the adaptation to high‐concentrate diets, decreased digestive disorders (rumen acidosis and diarrhoea) and increased BW gain. Once adapted to the diet, inoculated animals exhibited higher rumen VFA concentration (+16%), blood glucose (+28%), rumen papillae width (+43%) and increased expression of rumen epithelium genes involved in the cell proliferation (Cyclin 1), VFA absorption (MCCT1) and VFA metabolism (HMGCL), suggesting an enhanced energy uptake capacity. Inoculation with autoclaved rumen fluid as source of VFA had lower long‐term effects compared to fresh inocula. No differences across treatments were noted for feed digestibility, N excretion, and microbial protein synthesis. In conclusion, promoting greater rumen microbial diversity is a desirable strategy to prevent digestive disorders during the adaptation process to high‐concentrate diets, having minor effects once the animals are adapted to this diet.
000164122 536__ $$9info:eu-repo/grantAgreement/ES/AEI/AGL2017-86938-R$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2019-027764-I/AEI/10.13039/501100011033
000164122 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000164122 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000164122 700__ $$aPalma-Hidalgo, Juan Manuel
000164122 700__ $$aYáñez-Ruiz, David R.
000164122 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000164122 773__ $$g(2025), [21 pp.]$$pJ. anim. physiol. anim. nutr.$$tJournal of Animal Physiology and Animal Nutrition$$x0931-2439
000164122 8564_ $$s1107690$$uhttps://zaguan.unizar.es/record/164122/files/texto_completo.pdf$$yVersión publicada
000164122 8564_ $$s2219317$$uhttps://zaguan.unizar.es/record/164122/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000164122 909CO $$ooai:zaguan.unizar.es:164122$$particulos$$pdriver
000164122 951__ $$a2025-11-21-14:26:58
000164122 980__ $$aARTICLE