000164130 001__ 164130
000164130 005__ 20251121161352.0
000164130 0247_ $$2doi$$a10.1007/s11259-025-10948-w
000164130 0248_ $$2sideral$$a146292
000164130 037__ $$aART-2025-146292
000164130 041__ $$aeng
000164130 100__ $$aMartínez-Rodrigo, Lydia
000164130 245__ $$aEvaluating DAPI stain to assess sperm membrane integrity by flow cytometry in livestock species
000164130 260__ $$c2025
000164130 5060_ $$aAccess copy available to the general public$$fUnrestricted
000164130 5203_ $$aSperm viability is one of the key in vitro parameters of sperm quality closely related to fertility. Typically, sperm viability is assessed by flow cytometry (FC) with propidium iodide (PI) as the fluorescent stain. However, as PI emits in the red spectrum, it can overlap with other commonly used fluorochromes. Utilizing a blue viability stain, such as DAPI, for semen evaluation would free the red channel, enabling the use of additional fluorochromes and expanding analytical possibilities. This study aimed to evaluate the efficiency of DAPI as a viability stain for FC in three livestock species (goat, rabbit, and cattle), for which three experiments were conducted. First, the effect of incubation time on sperm viability rates was examined with DAPI and PI. Results indicated that incubation time did not affect the sperm permeability when DAPI and PI were employed. Second, the efficiency of DAPI as a viability indicator on different livestock species was studied. Viability rates measured with DAPI in goat, rabbit, and bull sperm samples showed significant correlation with PI measurements, showing values of 0.87, 0.86, and 0.61, respectively (P < 0.01). Third, the effect of intrasample variation on viability rate was analyzed. When bovine sperm samples were stained simultaneously with DAPI and PI, the correlation between the two protocols was 0.83. In conclusion, DAPI is an alternative to PI for assessing sperm viability in livestock species, whilst also highlighting the importance of possible inter-species differences.
000164130 536__ $$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1
000164130 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000164130 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000164130 700__ $$aGacem, Sabrina
000164130 700__ $$aBrüggemann, Maren
000164130 700__ $$aPinto, Sandra
000164130 700__ $$aMocé, Eva
000164130 700__ $$aSerrano-Jara, Daniel
000164130 700__ $$aGarcía, M. Luz
000164130 700__ $$0(orcid)0000-0001-5316-1703$$aYániz, Jesús L.$$uUniversidad de Zaragoza
000164130 700__ $$aSilvestre, Miguel A.
000164130 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000164130 773__ $$g49 (2025), 357 [13 pp.]$$pVet. res. commun.$$tVeterinary Research Communications$$x0165-7380
000164130 8564_ $$s1799840$$uhttps://zaguan.unizar.es/record/164130/files/texto_completo.pdf$$yVersión publicada
000164130 8564_ $$s2263367$$uhttps://zaguan.unizar.es/record/164130/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000164130 909CO $$ooai:zaguan.unizar.es:164130$$particulos$$pdriver
000164130 951__ $$a2025-11-21-14:27:07
000164130 980__ $$aARTICLE