000165094 001__ 165094
000165094 005__ 20251212165957.0
000165094 0247_ $$2doi$$a10.31989/ffhd.v15i11.1774
000165094 0248_ $$2sideral$$a146653
000165094 037__ $$aART-2025-146653
000165094 041__ $$aeng
000165094 100__ $$0(orcid)0000-0001-5573-6144$$aLayunta, Elena$$uUniversidad de Zaragoza
000165094 245__ $$aDifferential protective effects of whole and skimmed whey on LPS-induced barrier dysfunction and inflammation in Caco-2/TC7 cells
000165094 260__ $$c2025
000165094 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165094 5203_ $$aBackground: Lipopolysaccharide (LPS) from Gram-negative bacteria disrupts intestinal epithelial integrity and promotes inflammation, contributing to the pathogenesis of gastrointestinal disorders. Dairy-based whey products are rich in bioactive compounds with known barrier-protective and immunomodulatory properties.
Objective: This study aimed to evaluate the effects of two types of bovine whey — bovine whole whey (BWW) and bovine skimmed whey (BSW) — on LPS-induced barrier dysfunction and inflammation in the intestinal in vitro model Caco-2/TC7 cells.
Methods: Caco-2/TC7 cell line was exposed to 20 µg/ml LPS for 24h to induce epithelial barrier disruption. Additionally, cells were treated with 5 mg/ml BWW or 5 mg/ml BSW alone, or in combination with 20 µg/ml LPS. Transepithelial electrical resistance (TEER), FITC-dextran permeability, and mRNA expression of tight junction (TJ) proteins (ZO-1, claudin-1, claudin-3, occludin) and inflammation-related genes (IL-1R, COX-2, iNOS) were assessed.
Results: LPS significantly reduced TEER without increasing macromolecular permeability or altering TJ gene expression. Both whey fractions restored TEER to control levels, with BSW also enhancing baseline barrier integrity. BWW and BSW upregulated ZO-1 and claudin-1 mRNA expression, though claudin-1 was downregulated when BSW was combined with LPS. Importantly, only BWW normalized LPS-induced overexpression of IL-1R, COX-2, and iNOS, indicating better anti-inflammatory properties likely due to its retained lipid components.
Conclusion: Both whey fractions reinforce epithelial barrier function under LPS challenge, but only whole whey (BWW) provides dual protection by also attenuating inflammatory responses. These findings support the potential of whole whey as a functional food ingredient for intestinal health by targeting both physical and immunological aspects of barrier dysfunction.
Keywords: bovine whey, LPS, intestinal permeability, tight junction proteins, inflammation
000165094 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A20-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-139104OB-I00
000165094 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000165094 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165094 700__ $$aGarcía, Laura$$uUniversidad de Zaragoza
000165094 700__ $$aGimeno, Sofía$$uUniversidad de Zaragoza
000165094 700__ $$aMartínez, Jaime$$uUniversidad de Zaragoza
000165094 700__ $$0(orcid)0000-0001-5964-823X$$aSánchez, Lourdes$$uUniversidad de Zaragoza
000165094 700__ $$0(orcid)0000-0002-5306-9365$$aGras, Laura$$uUniversidad de Zaragoza
000165094 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000165094 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000165094 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000165094 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000165094 773__ $$g15, 11 (2025), 867-881$$tFunctional Foods in Health and Disease$$x2378-7007
000165094 8564_ $$s2431059$$uhttps://zaguan.unizar.es/record/165094/files/texto_completo.pdf$$yVersión publicada
000165094 8564_ $$s1881026$$uhttps://zaguan.unizar.es/record/165094/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165094 909CO $$ooai:zaguan.unizar.es:165094$$particulos$$pdriver
000165094 951__ $$a2025-12-12-14:42:33
000165094 980__ $$aARTICLE