000165154 001__ 165154
000165154 005__ 20251212165958.0
000165154 0247_ $$2doi$$a10.1021/acsami.5c18555
000165154 0248_ $$2sideral$$a146591
000165154 037__ $$aART-2025-146591
000165154 041__ $$aeng
000165154 100__ $$aBesbinar, Omur
000165154 245__ $$aCell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights
000165154 260__ $$c2025
000165154 5203_ $$aExosomes, nanoscale extracellular vesicles, have emerged as promising carriers in drug delivery due to their ability to bypass biological barriers, low toxicity, high stability, and intrinsic targeting capabilities. Mesenchymal stem-cell-derived exosomes (EXOMSC), with their natural tropism toward the tumor microenvironment, offer an ideal platform for enhancing therapeutic cargo delivery. In this study, we demonstrate an approach where red-emission chlorophyll-based carbon dots (Chl-CDs) were encapsulated within EXOMSC through a cell-driven uptake mechanism, creating CD@EXOMSC. These exosomes achieved superior photodynamic therapy (PDT) efficacy, requiring 40 times less nanomaterial compared to freestanding Chl-CDs. Mechanistic insights from glioblastoma cell miRNA profiling revealed that the enhanced efficacy was mediated by the regulation of efflux transporter genes, oxidative stress responses, and endocytosis pathways. This work highlights the synergistic potential of combining photosensitizers and miRNA-rich exosomes to achieve targeted and sustained therapeutic delivery, paving the way for a multifaceted approach in cancer therapy.
000165154 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000165154 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165154 700__ $$aUyar, Recep
000165154 700__ $$aKirbas Cilingir, Emel
000165154 700__ $$aMartín-Pardillos, Ana$$uUniversidad de Zaragoza
000165154 700__ $$aHueso, Jose L.
000165154 700__ $$aCeylan, Ahmet
000165154 700__ $$aOzgenc, Ozge
000165154 700__ $$aEkim, Okan
000165154 700__ $$aUnal, Mehmet Altay
000165154 700__ $$aAri, Fikret
000165154 700__ $$aLeblanc, Roger M.
000165154 700__ $$aSantamaria, Jesus
000165154 700__ $$aYilmazer, Acelya
000165154 7102_ $$11002$$2807$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Toxicología
000165154 773__ $$g(2025), [16 pp.]$$pACS appl. mater. interfaces$$tACS applied materials & interfaces$$x1944-8244
000165154 8564_ $$s12460950$$uhttps://zaguan.unizar.es/record/165154/files/texto_completo.pdf$$yVersión publicada
000165154 8564_ $$s3125752$$uhttps://zaguan.unizar.es/record/165154/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165154 909CO $$ooai:zaguan.unizar.es:165154$$particulos$$pdriver
000165154 951__ $$a2025-12-12-14:43:55
000165154 980__ $$aARTICLE