165154 20260227124019.0 doi 10.1021/acsami.5c18555 sideral 146591 ART-2025-146591 eng Besbinar, Omur Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights 2025 Exosomes, nanoscale extracellular vesicles, have emerged as promising carriers in drug delivery due to their ability to bypass biological barriers, low toxicity, high stability, and intrinsic targeting capabilities. Mesenchymal stem-cell-derived exosomes (EXOMSC), with their natural tropism toward the tumor microenvironment, offer an ideal platform for enhancing therapeutic cargo delivery. In this study, we demonstrate an approach where red-emission chlorophyll-based carbon dots (Chl-CDs) were encapsulated within EXOMSC through a cell-driven uptake mechanism, creating CD@EXOMSC. These exosomes achieved superior photodynamic therapy (PDT) efficacy, requiring 40 times less nanomaterial compared to freestanding Chl-CDs. Mechanistic insights from glioblastoma cell miRNA profiling revealed that the enhanced efficacy was mediated by the regulation of efflux transporter genes, oxidative stress responses, and endocytosis pathways. This work highlights the synergistic potential of combining photosensitizers and miRNA-rich exosomes to achieve targeted and sustained therapeutic delivery, paving the way for a multifaceted approach in cancer therapy. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Uyar, Recep Kirbas Cilingir, Emel Martín-Pardillos, Ana Universidad de Zaragoza Hueso, Jose L. Ceylan, Ahmet Ozgenc, Ozge Ekim, Okan Unal, Mehmet Altay Ari, Fikret Leblanc, Roger M. Santamaria, Jesus Yilmazer, Acelya 1002 807 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Toxicología (2025), [16 pp.] ACS appl. mater. interfaces ACS applied materials & interfaces 1944-8244 12521490 http://zaguan.unizar.es/record/165154/files/texto_completo.pdf Versión publicada 3221916 http://zaguan.unizar.es/record/165154/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:165154 articulos driver 2026-02-27-12:39:23 ARTICLE