000165250 001__ 165250
000165250 005__ 20251219174252.0
000165250 0247_ $$2doi$$a10.1016/j.isci.2025.113992
000165250 0248_ $$2sideral$$a146984
000165250 037__ $$aART-2025-146984
000165250 041__ $$aeng
000165250 100__ $$aOlaizola-Rodrigo, Claudia
000165250 245__ $$aA review of organ-on-chip fabrication methods: From early developments to overcoming inert barriers
000165250 260__ $$c2025
000165250 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165250 5203_ $$aOrgan-on-chip (OOC) systems represent a significant advance in the effort to replicate human physiology in vitro, providing versatile models that extend beyond the limitations of static culture and animal experimentation. The fabrication methods and materials underlying these platforms play a decisive role in determining their structural accuracy, biological relevance, and potential for large-scale adoption. This review surveys the historical progression of OOC manufacturing, beginning with established microfabrication techniques such as photolithography, soft lithography, hot embossing, microinjection molding, and xurography and extending to recent innovations in additive manufacturing. Particular attention is given to emerging barrier-free strategies, including laminar flow patterning, hydrogel photopatterning, phaseguide design, and surface treatment patterning that permit direct tissue-tissue communication. These methods aim to enhance biomimicry by reducing artificial interfaces, thereby improving the simulation of intercellular gradients, multicellular crosstalk, and pathophysiological dynamics. Alongside their benefits, the review discusses the practical challenges these approaches introduce in terms of reproducibility, throughput, and scalability. By integrating advances in materials science, fabrication techniques, and microphysiological design, this review highlights the potential of next-generation OOC devices to provide predictive, translationally relevant platforms that narrow the divide between preclinical experimentation and clinical application.
000165250 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/DIN2020-011544
000165250 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000165250 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165250 700__ $$0(orcid)0000-0001-6786-2041$$aBayona, Clara
000165250 700__ $$0(orcid)0000-0003-0156-4230$$aOliván, Sara$$uUniversidad de Zaragoza
000165250 700__ $$aMonge, Rosa
000165250 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000165250 773__ $$g28, 12 (2025), 113992 [43 pp.]$$piScience$$tISCIENCE$$x2589-0042
000165250 8564_ $$s3551162$$uhttps://zaguan.unizar.es/record/165250/files/texto_completo.pdf$$yVersión publicada
000165250 8564_ $$s3531838$$uhttps://zaguan.unizar.es/record/165250/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165250 909CO $$ooai:zaguan.unizar.es:165250$$particulos$$pdriver
000165250 951__ $$a2025-12-19-14:43:48
000165250 980__ $$aARTICLE