165264 20251219174252.0 doi 10.1021/acs.jproteome.3c00461 sideral 136287 ART-2023-136287 eng Gacem, Sabrina Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm–Oocyte Interaction 2023 The prediction of male or semen fertility potential remains a persistent challenge that has yet to be fully resolved. This work analyzed several in vitro parameters and proteome of spermatozoa in bulls cataloged as high- (HF; n = 5) and low-field (LF; n = 5) fertility after more than a thousand artificial inseminations. Sperm motility was evaluated by computer-assisted sperm analysis. Sperm viability, mitochondrial membrane potential (MMP) and reactive oxygen species (mROS) of spermatozoa were assessed by flow cytometry. Proteome was evaluated by the SWATH-MS procedure. Spermatozoa of HF bulls showed significantly higher total motility than the LF group (41.4% vs 29.7%). Rates of healthy sperm (live, high MMP, and low mROS) for HF and LF bull groups were 49% and 43%, respectively (p > 0.05). Spermatozoa of HF bulls showed a higher presence of differentially abundant proteins (DAPs) related to both energy production (COX7C), mainly the OXPHOS pathway, and the development of structures linked with the motility process (TPPP2, SSMEM1, and SPAG16). Furthermore, we observed that equatorin (EQTN), together with other DAPs related to the interaction with the oocyte, was overrepresented in HF bull spermatozoa. The biological processes related to protein processing, catabolism, and protein folding were found to be overrepresented in LF bull sperm in which the HSP90AA1 chaperone was identified as the most DAP. Data are available via ProteomeXchange with identifier PXD042286. info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1 info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.8 2023 BIOCHEMICAL RESEARCH METHODS 15 / 85 = 0.176 2023 Q1 T1 1.299 2023 Chemistry (miscellaneous) 2023 Q1 Biochemistry 2023 Q1 9.0 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Castello-Ruiz, María Hidalgo, Carlos O. Tamargo, Carolina Santolaria, Pilar Universidad de Zaragoza (orcid)0000-0001-8991-325X Soler, Carles Yániz, Jesús L. Universidad de Zaragoza (orcid)0000-0001-5316-1703 Silvestre, Miguel A. 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 22, 11 (2023), 3607-3624 J. proteome res. JOURNAL OF PROTEOME RESEARCH 1535-3893 5465595 http://zaguan.unizar.es/record/165264/files/texto_completo.pdf Versión publicada 2927818 http://zaguan.unizar.es/record/165264/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:165264 articulos driver 2025-12-19-14:44:02 ARTICLE