000165290 001__ 165290
000165290 005__ 20251219174252.0
000165290 0247_ $$2doi$$a10.1111/nph.70360
000165290 0248_ $$2sideral$$a145864
000165290 037__ $$aART-2025-145864
000165290 041__ $$aeng
000165290 100__ $$aCui, Xingyong
000165290 245__ $$aAncient hybridization drives arid adaptation and species diversification in "Caragana" (Fabaceae)
000165290 260__ $$c2025
000165290 5203_ $$aSummary
The genus Caragana (Fabaceae), comprising predominantly xerophytic shrubs, is ecologically important in arid ecosystems across Asia and Eastern Europe. Hybridization is increasingly recognized as a critical evolutionary mechanism, yet its role in arid adaptation and lineage diversification remains underexplored. Here, we test the hypothesis that ancient hybridization‐facilitated ecological expansion and diversification in Caragana.
We generated a chromosome‐level genome assembly for Caragana arborescens and conducted genome‐wide resequencing of 98 individuals across 73 species, representing the full taxonomic breadth of the genus. Phylogenomic reconstructions and reticulation analyses (QuIBL, HyDe, PhyloNet, and TreeMix) uncovered pervasive ancient hybridization, particularly between sections Caragana and Calophaca, which gave rise to five descendant lineages. These hybridization events coincided with late Oligocene aridification and were temporally associated with sharp increases in diversification rates.
Introgressed genomic regions were significantly enriched for genes involved in drought tolerance, including those regulating root development, osmoregulation, and hormone signaling. Key gene families, such as late embryogenesis abundant (LEA), sulfate transporters (SULTR), NAM‐ATAF‐CUC (NAC), and nitrate transporter 1 / peptide transporter family (NPF), exhibited duplications and evolutionary rate shifts in hybrid lineages. Furthermore, Aridity Index data confirmed that hybrid lineages occupy more arid habitats than either parent, highlighting a link between introgression and ecological breadth.
Our findings demonstrate that ancient hybridization served as a catalyst for adaptive innovation and diversification in Caragana, offering a genomic mechanism for rapid radiation in response to climatic aridification. This study underscores the evolutionary significance of hybridization in enabling plant lineages to colonize and thrive in extreme environments.
000165290 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000165290 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165290 700__ $$aLi, Enze
000165290 700__ $$aHe, Jian
000165290 700__ $$aWang, Yachao
000165290 700__ $$aShang, Ce
000165290 700__ $$aZhong, Bojian
000165290 700__ $$0(orcid)0000-0001-5658-8411$$aViruel, Juan$$uUniversidad de Zaragoza
000165290 700__ $$aDong, Wenpan
000165290 700__ $$aZhang, Zhixiang
000165290 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica
000165290 773__ $$g247, 5 (2025), 2454-2472$$pNew phytol.$$tNew Phytologist$$x0028-646X
000165290 787__ $$tAdaptive-evolution-of-the-Caragana$$whttps://github.com/cuixylab/Adaptive-evolution-of-the-Caragana
000165290 8564_ $$s7016222$$uhttps://zaguan.unizar.es/record/165290/files/texto_completo.pdf$$yVersión publicada
000165290 8564_ $$s3013396$$uhttps://zaguan.unizar.es/record/165290/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165290 909CO $$ooai:zaguan.unizar.es:165290$$particulos$$pdriver
000165290 951__ $$a2025-12-19-14:44:30
000165290 980__ $$aARTICLE