000165364 001__ 165364
000165364 005__ 20260107201858.0
000165364 0247_ $$2doi$$a10.1021/acsami.3c07367
000165364 0248_ $$2sideral$$a135307
000165364 037__ $$aART-2023-135307
000165364 041__ $$aeng
000165364 100__ $$aMiranda Calderon, Laura Gabriela$$uUniversidad de Zaragoza
000165364 245__ $$aAntibody-Functionalized Polymer Nanoparticles for Targeted Antibiotic Delivery in Models of Pathogenic Bacteria Infecting Human Macrophages
000165364 260__ $$c2023
000165364 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165364 5203_ $$aThe efficacy of antibody-functionalized poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles (NPs), prepared by nanoprecipitation, carrying rifampicin (RIF) against planktonic, sessile, and intracellular Staphylococcus aureus and Escherichia coli is reported here. A biotinylated anti-S. aureus polyclonal antibody, which binds to structural antigens of the whole bacterium, was functionalized on the surface of RIF-loaded PLGA-based NPs by using the high-affinity avidin–biotin complex. This general strategy allows the binding of commercially available biotinylated antibodies. Coculture models of S. aureus ATCC 25923 and Escherichia coli S17 were used to demonstrate the preferential selectivity of the antibody-functionalized NPs against the Gram-positive bacterium only. At 0.2 µg/mL, complete S. aureus eradication was observed for the antibody-functionalized RIF-loaded NPs, whereas only a 5-log reduction was observed for the nontargeted RIF-loaded NPs. S. aureus is a commensal facultative pathogen having part of its live cycle intracellularly in both phagocytic and nonphagocytic cells. Those intracellular bacterial persisters, named small colony variants, have been postulated as reservoirs of relapsed episodes of infection and consequent treatment failure. At 0.5 µg/mL, the RIF-loaded NPs reduced in 2-log intracellular S. aureus-infecting human macrophages. The ability of those antibody-functionalized nanoparticles to prevent biofilm formation or to reduce the bacterial burden in already-formed mature biofilms is also reported here using S. aureus and E. coli single and cocultured biofilms. In the prevention of S. aureus biofilm formation, the antibody-functionalized NPs exerted a superior inhibition of bacterial growth (up to 2 logs) compared to the nonfunctionalized ones. This study demonstrates the selectivity of the synthesized immunonanoparticles and their antimicrobial efficacy in different scenarios, including planktonic cultures, sessile conditions, and even against intracellular infective pathogens.
000165364 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/MS19-00092$$9info:eu-repo/grantAgreement/ES/MICINN/PDC2021-121405-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-113987RB-I00
000165364 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000165364 590__ $$a8.5$$b2023
000165364 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b28 / 141 = 0.199$$c2023$$dQ1$$eT1
000165364 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b68 / 439 = 0.155$$c2023$$dQ1$$eT1
000165364 592__ $$a2.058$$b2023
000165364 593__ $$aMaterials Science (miscellaneous)$$c2023$$dQ1
000165364 593__ $$aNanoscience and Nanotechnology$$c2023$$dQ1
000165364 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1
000165364 594__ $$a16.0$$b2023
000165364 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000165364 700__ $$0(orcid)0000-0002-9324-0446$$aAlejo, Teresa
000165364 700__ $$aSantos, Sabas
000165364 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia
000165364 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, Silvia$$uUniversidad de Zaragoza
000165364 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000165364 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000165364 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente
000165364 773__ $$g15, 34 (2023), 40213-40227$$pACS appl. mater. interfaces$$tACS applied materials & interfaces$$x1944-8244
000165364 8564_ $$s5599041$$uhttps://zaguan.unizar.es/record/165364/files/texto_completo.pdf$$yPostprint
000165364 8564_ $$s1712234$$uhttps://zaguan.unizar.es/record/165364/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000165364 909CO $$ooai:zaguan.unizar.es:165364$$particulos$$pdriver
000165364 951__ $$a2026-01-07-18:53:12
000165364 980__ $$aARTICLE