000165403 001__ 165403
000165403 005__ 20260108143203.0
000165403 0247_ $$2doi$$a10.1186/cc8208
000165403 0248_ $$2sideral$$a67971
000165403 037__ $$aART-2009-67971
000165403 041__ $$aeng
000165403 100__ $$aBermejo-Martin, Jesús F.
000165403 245__ $$aTh1 and Th17 hypercytokinemia as early host response signature in severe pandemic influenza
000165403 260__ $$c2009
000165403 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165403 5203_ $$aIntroduction: Human host immune response following infection with the new variant of A/H1N1 pandemic influenza virus (nvH1N1) is poorly understood. We utilize here systemic cytokine and antibody levels in evaluating differences in early immune response in both mild and severe patients infected with nvH1N1.
Methods: We profiled 29 cytokines and chemokines and evaluated the haemagglutination inhibition activity as quantitative and qualitative measurements of host immune responses in serum obtained during the first five days after symptoms onset, in two cohorts of nvH1N1 infected patients. Severe patients required hospitalization (n = 20), due to respiratory insufficiency (10 of them were admitted to the intensive care unit), while mild patients had exclusively flu-like symptoms (n = 15). A group of healthy donors was included as control (n = 15). Differences in levels of mediators between groups were assessed by using the non parametric U-Mann Whitney test. Association between variables was determined by calculating the Spearman correlation coefficient. Viral load was performed in serum by using real-time PCR targeting the neuraminidase gene.
Results: Increased levels of innate-immunity mediators (IP-10, MCP-1, MIP-1ß), and the absence of anti-nvH1N1 antibodies, characterized the early response to nvH1N1 infection in both hospitalized and mild patients. High systemic levels of type-II interferon (IFN-¿) and also of a group of mediators involved in the development of T-helper 17 (IL-8, IL-9, IL-17, IL-6) and T-helper 1 (TNF-a, IL-15, IL-12p70) responses were exclusively found in hospitalized patients. IL-15, IL-12p70, IL-6 constituted a hallmark of critical illness in our study. A significant inverse association was found between IL-6, IL-8 and PaO2 in critical patients.
Conclusions: While infection with the nvH1N1 induces a typical innate response in both mild and severe patients, severe disease with respiratory involvement is characterized by early secretion of Th17 and Th1 cytokines usually associated with cell mediated immunity but also commonly linked to the pathogenesis of autoimmune/inflammatory diseases. The exact role of Th1 and Th17 mediators in the evolution of nvH1N1 mild and severe disease merits further investigation as to the detrimental or beneficial role these cytokines play in severe illness.
000165403 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000165403 590__ $$a4.931$$b2009
000165403 591__ $$aCRITICAL CARE MEDICINE$$b4 / 22 = 0.182$$c2009$$dQ1$$eT1
000165403 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165403 700__ $$aOrtiz de Lejarazu, Raul
000165403 700__ $$aPumarola, Tomás
000165403 700__ $$aRello, Jordi
000165403 700__ $$aAlmansa, Raquel
000165403 700__ $$aRamírez, Paula
000165403 700__ $$aMartin-Loeches, Ignacio
000165403 700__ $$aVarillas, Savid
000165403 700__ $$aGallegos, Maria Carmen
000165403 700__ $$0(orcid)0000-0002-8068-5016$$aSerón Arbeloa, Carlos$$uUniversidad de Zaragoza
000165403 700__ $$aMicheloud,Daniela
000165403 700__ $$aGomez, José Manuel
000165403 700__ $$aTenorio-Abreu, Alberto
000165403 700__ $$aRamos, María José
000165403 700__ $$aMolina, Lourdes
000165403 700__ $$aHuidobro, Samantha
000165403 700__ $$aSanchez, Elia
000165403 700__ $$aGordón, Mónica
000165403 700__ $$aFernández, Victoria
000165403 700__ $$aCastillo, Alberto del
000165403 700__ $$aMarcos, Maria Ángeles
000165403 700__ $$aVillanueva, Beatriz
000165403 700__ $$aLópez, Carlos Javier
000165403 700__ $$aRodríguez-Domínguez, Maria
000165403 700__ $$aGalan, Juan Carlos
000165403 700__ $$aCantón, Rafael
000165403 700__ $$aLietor, Aurora
000165403 700__ $$aRojo, Silvia
000165403 700__ $$aEiros, JM:
000165403 700__ $$aHinojosa, Carmen
000165403 700__ $$aGonzalez, Isabel
000165403 700__ $$aTorner, Nuria
000165403 700__ $$aBanner, David
000165403 700__ $$aLeon, Alberto
000165403 700__ $$aCuesta, Pablo
000165403 700__ $$aRowe, Thomas
000165403 700__ $$aKelvin, David
000165403 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000165403 773__ $$g13, 6 (2009), R201 [11 pp.]$$pCrit. care$$tCritical care$$x1364-8535
000165403 8564_ $$s1124099$$uhttps://zaguan.unizar.es/record/165403/files/texto_completo.pdf$$yVersión publicada
000165403 8564_ $$s2766744$$uhttps://zaguan.unizar.es/record/165403/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165403 909CO $$ooai:zaguan.unizar.es:165403$$particulos$$pdriver
000165403 951__ $$a2026-01-08-14:09:55
000165403 980__ $$aARTICLE