165458 20260108143204.0 doi 10.3390/cancers12040920 sideral 146778 ART-2020-146778 eng Hernández-Reséndiz, Ileana Inhibition of Kv10.1 Channels Sensitizes Mitochondria of Cancer Cells to Antimetabolic Agents 2020 Reprogramming of energy metabolism constitutes one of the hallmarks of cancer and is, therefore, an emerging therapeutic target. We describe here that the potassium channel Kv10.1, which is frequently overexpressed in primary and metastatic cancer, and has been proposed a therapeutic target, participates in metabolic adaptation of cancer cells through regulation of mitochondrial dynamics. We used biochemical and cell biological techniques, live cell imaging and high-resolution microscopy, among other approaches, to study the impact of Kv10.1 on the regulation of mitochondrial stability. Inhibition of Kv10.1 expression or function led to mitochondrial fragmentation, increase in reactive oxygen species and increased autophagy. Cells with endogenous overexpression of Kv10.1 were also more sensitive to mitochondrial metabolism inhibitors than cells with low expression, indicating that they are more dependent on mitochondrial function. Consistently, a combined therapy using functional monoclonal antibodies for Kv10.1 and mitochondrial metabolism inhibitors resulted in enhanced efficacy of the inhibitors. Our data reveal a new mechanism regulated by Kv10.1 in cancer and a novel strategy to overcome drug resistance in cancers with a high expression of Kv10.1. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 6.639 2020 ONCOLOGY 51 / 241 = 0.212 2020 Q1 T1 1.818 2020 Oncology 2020 Q1 Cancer Research 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pacheu-Grau, David Universidad de Zaragoza (orcid)0000-0003-2645-3983 Sánchez, Araceli Pardo, Luis A. 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 12, 4 (2020), 920 [20 pp.] Cancers Cancers 2072-6694 8264149 http://zaguan.unizar.es/record/165458/files/texto_completo.pdf Versión publicada 2524611 http://zaguan.unizar.es/record/165458/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:165458 articulos driver 2026-01-08-14:11:00 ARTICLE