000165551 001__ 165551
000165551 005__ 20260112132215.0
000165551 0247_ $$2doi$$a10.1016/j.fawpar.2025.e00308
000165551 0248_ $$2sideral$$a147236
000165551 037__ $$aART-2026-147236
000165551 041__ $$aeng
000165551 100__ $$0(orcid)0000-0001-5053-8309$$aBerdejo, Daniel$$uUniversidad de Zaragoza
000165551 245__ $$aOccurrence of Toxoplasma gondii in Iberian pork and its association with pig seropositivity
000165551 260__ $$c2026
000165551 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165551 5203_ $$aPork is recognized as a major source of Toxoplasma gondii infection in humans. Although the potential association between seropositivity in white pigs and the presence of T. gondii in their meat has been investigated, corresponding information on the Iberian pig breed is still limited. In this study, we investigated the presence of T. gondii in Iberian pork and assessed its correlation with individual serological profiles to evaluate whether antibody titres can serve as indicators of meat contamination. We tested the sera of 238 Iberian pigs from three southwestern Spanish provinces (Badajoz, Cáceres, and Córdoba) using an indirect immunofluorescence assay (IFA), and analyzed matched diaphragm samples by quantitative PCR (qPCR) for T. gondii DNA detection. Serological analysis revealed an overall seropositivity rate of 46.22 %, with significant regional differences (p ≤ 0.050). Córdoba exhibited the highest seropositivity (57.89 %), followed by Cáceres (48.38 %) and Badajoz (35.71 %). Concurrently, T. gondii DNA was present in 14.29 % of the diaphragm samples, with parasite loads ranging from 78.56 to 219.09 parasites/g. A statistically significant correlation (p ≤ 0.001) was observed between grouped IFA titres (<1:20, 1:20–1:40, ≥1:80) and qPCR positivity in the corresponding diaphragm samples. Notably, the proportion of animals with titres ≥1:80 closely matched the rate of qPCR-positive meat samples. We concluded that this serological threshold can serve as an effective screening tool to discriminate animals that are at a higher risk of harboring the parasite, thereby improving food safety within the HACCP-based safety system at the slaughterhouse and in the meat industry.
000165551 536__ $$9info:eu-repo/grantAgreement/ES/INIA/RTA2014-00024-C04-02
000165551 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000165551 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165551 700__ $$0(orcid)0000-0003-2675-6706$$aNieto, Paula
000165551 700__ $$0(orcid)0000-0003-1417-272X$$aGracia, Mª. Jesús
000165551 700__ $$0(orcid)0000-0002-1204-4356$$ade Blas, Ignacio$$uUniversidad de Zaragoza
000165551 700__ $$0(orcid)0000-0002-2147-9263$$aRemón, Sara$$uUniversidad de Zaragoza
000165551 700__ $$0(orcid)0000-0002-6460-1388$$aLázaro, Regina$$uUniversidad de Zaragoza
000165551 700__ $$0(orcid)0000-0002-1668-4940$$aBayarri, Susana$$uUniversidad de Zaragoza
000165551 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000165551 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000165551 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000165551 773__ $$g42 (2026), e00308 [9 pp.]$$tFood and Waterborne Parasitology$$x2405-6766
000165551 8564_ $$s823877$$uhttps://zaguan.unizar.es/record/165551/files/texto_completo.pdf$$yVersión publicada
000165551 8564_ $$s1956274$$uhttps://zaguan.unizar.es/record/165551/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165551 909CO $$ooai:zaguan.unizar.es:165551$$particulos$$pdriver
000165551 951__ $$a2026-01-12-11:09:55
000165551 980__ $$aARTICLE