000165665 001__ 165665
000165665 005__ 20260113234334.0
000165665 0247_ $$2doi$$a10.1016/j.prevetmed.2020.105196
000165665 0248_ $$2sideral$$a121243
000165665 037__ $$aART-2020-121243
000165665 041__ $$aeng
000165665 100__ $$aBenito, A.A.
000165665 245__ $$aOccurrence and genetic diversity of rotavirus A in faeces of diarrheic calves submitted to a veterinary laboratory in Spain
000165665 260__ $$c2020
000165665 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165665 5203_ $$aA total of 237 faecal specimens from diarrheic calves younger than two months were collected and submitted for diagnosis of enteropathogens over a two-year period (2017–2018) to a veterinary laboratory. Samples originated from 193 dairy and beef farms in 29 provinces distributed throughout Spain, and were tested for the occurrence of three target enteric pathogens by reverse transcription real-time PCR (RT-qPCR): bovine rotavirus A (RVA), Cryptosporidium parvum and bovine coronavirus (BCoV). RT-PCR and nucleotide sequencing analysis were used to determine the G (VP7 gene) and P (VP4 gene) genotypes of 26 specimens positive for RVA. A total of 188 specimens (79.3 %) were positive for at least one of the three target enteric pathogens, and 101 samples (42.6 %) harbored mixed infections. The individual prevalence was 57.8 %, 50.6 % and 23.6 % for C. parvum, RVA and BCoV, respectively. Molecular analysis of selected RVA strains revealed the presence of the G6, G10, G3, P[5] and P[11] genotypes, with the combinations G6P[5] and G6P[11] being the most prevalent. Alignments of nucleotide sequences of the VP7 and VP4 markers showed a high frequency of single nucleotide polymorphisms (SNPs), with up to 294 SNPs found in 869bp of sequence at the G6 genotype (0.338 SNPs/nt), which reveals the extensive genetic diversity of RVA strains. Phylogenetic analysis of the VP7 gene of the G6 strains revealed four distinct lineages, with most strains clustering in the G6-IV lineage. The discrepancies between the RVA genotypes circulating in the sampled cattle farms and the genotypes contained in commercial vaccines currently available in Spain are discussed. We believe that this is the first study on the molecular characterization of rotavirus infecting cattle in Spain.
000165665 540__ $$9info:eu-repo/semantics/openAccess$$aby-nd$$uhttps://creativecommons.org/licenses/by-nd/4.0/deed.es
000165665 590__ $$a2.67$$b2020
000165665 591__ $$aVETERINARY SCIENCES$$b27 / 146 = 0.185$$c2020$$dQ1$$eT1
000165665 592__ $$a0.815$$b2020
000165665 593__ $$aFood Animals$$c2020$$dQ1
000165665 593__ $$aAnimal Science and Zoology$$c2020$$dQ1
000165665 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000165665 700__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, L.V.$$uUniversidad de Zaragoza
000165665 700__ $$aArnal, J.L.
000165665 700__ $$aBaselga, C.
000165665 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, J.$$uUniversidad de Zaragoza
000165665 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000165665 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000165665 773__ $$g185 (2020), 105196 [8 pp]$$pPrev. vet. med.$$tPREVENTIVE VETERINARY MEDICINE$$x0167-5877
000165665 8564_ $$s662378$$uhttps://zaguan.unizar.es/record/165665/files/texto_completo.pdf$$yPostprint
000165665 8564_ $$s818913$$uhttps://zaguan.unizar.es/record/165665/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000165665 909CO $$ooai:zaguan.unizar.es:165665$$particulos$$pdriver
000165665 951__ $$a2026-01-13-22:05:23
000165665 980__ $$aARTICLE