000165691 001__ 165691
000165691 005__ 20260113234334.0
000165691 0247_ $$2doi$$a10.1161/ATVBAHA.106.132266
000165691 0248_ $$2sideral$$a66931
000165691 037__ $$aART-2007-66931
000165691 041__ $$aeng
000165691 100__ $$aVilla-Bellosta, Ricardo
000165691 245__ $$aCharacterization of Phosphate Transport in Rat Vascular Smooth Muscle Cells: Implication for Vascular Calcification
000165691 260__ $$c2007
000165691 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165691 5203_ $$aObjective— Hyperphosphatemia and inorganic phosphate (Pi) transport by vascular smooth muscle cells (VSMCs) have been implicated in the pathogenesis of vascular calcification. The aim of this work has been to characterize Pi transport in VSMCs.
Methods and Results— Primary cultures of VSMCs express both high affinity Na-dependent and Na-independent components of Pi transport. Under physiological conditions both transport systems are saturated, show similar activity, and are inhibited by increasing pH. The Na-dependent transport is also weakly inhibited by phosphonoformic acid (PFA) (3.9 mmol/L IC50 at 0.05 mmol/L Pi). Real-time polymerase chain reaction shows that Pit1 and Pit2 are expressed to the same degree, and no other Pi transporters are significantly expressed. When expressed in Xenopus oocytes they are strictly Na-dependent, with high affinities for Pi, and are inhibited by increasing pH, but only weakly inhibited by PFA. We have used RNA interference to demonstrate that Pit1 and Pit2 are the transporters responsible for Na-dependent Pi transport in VSMCs.
Conclusions— Taken together these novel findings suggest new roles of Pi transport in the pathogenesis of VC and have implications as potential future clinical targets.
000165691 536__ $$9info:eu-repo/grantAgreement/ES/MEC/BFU2016-06284-BFI
000165691 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000165691 590__ $$a7.221$$b2007
000165691 591__ $$aPERIPHERAL VASCULAR DISEASE$$b3 / 54 = 0.056$$c2007$$dQ1$$eT1
000165691 591__ $$aHEMATOLOGY$$b5 / 62 = 0.081$$c2007$$dQ1$$eT1
000165691 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000165691 700__ $$aBogaert, Yolanda E.
000165691 700__ $$aLevi, Moshe
000165691 700__ $$0(orcid)0000-0003-3457-323X$$aSorribas, Víctor$$uUniversidad de Zaragoza
000165691 7102_ $$11000$$2807$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Toxicología
000165691 773__ $$g27, 5 (2007), 1030-1036$$pArterioscler. thromb. vasc. biol.$$tArteriosclerosis, Thrombosis, and Vascular Biology$$x1079-5642
000165691 8564_ $$s403767$$uhttps://zaguan.unizar.es/record/165691/files/texto_completo.pdf$$yPostprint
000165691 8564_ $$s919715$$uhttps://zaguan.unizar.es/record/165691/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000165691 909CO $$ooai:zaguan.unizar.es:165691$$particulos$$pdriver
000165691 951__ $$a2026-01-13-22:06:03
000165691 980__ $$aARTICLE