165691 20260113234334.0 doi 10.1161/ATVBAHA.106.132266 sideral 66931 ART-2007-66931 eng Villa-Bellosta, Ricardo Characterization of Phosphate Transport in Rat Vascular Smooth Muscle Cells: Implication for Vascular Calcification 2007 Objective— Hyperphosphatemia and inorganic phosphate (Pi) transport by vascular smooth muscle cells (VSMCs) have been implicated in the pathogenesis of vascular calcification. The aim of this work has been to characterize Pi transport in VSMCs. Methods and Results— Primary cultures of VSMCs express both high affinity Na-dependent and Na-independent components of Pi transport. Under physiological conditions both transport systems are saturated, show similar activity, and are inhibited by increasing pH. The Na-dependent transport is also weakly inhibited by phosphonoformic acid (PFA) (3.9 mmol/L IC50 at 0.05 mmol/L Pi). Real-time polymerase chain reaction shows that Pit1 and Pit2 are expressed to the same degree, and no other Pi transporters are significantly expressed. When expressed in Xenopus oocytes they are strictly Na-dependent, with high affinities for Pi, and are inhibited by increasing pH, but only weakly inhibited by PFA. We have used RNA interference to demonstrate that Pit1 and Pit2 are the transporters responsible for Na-dependent Pi transport in VSMCs. Conclusions— Taken together these novel findings suggest new roles of Pi transport in the pathogenesis of VC and have implications as potential future clinical targets. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/MEC/BFU2016-06284-BFI info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 7.221 2007 PERIPHERAL VASCULAR DISEASE 3 / 54 = 0.056 2007 Q1 T1 HEMATOLOGY 5 / 62 = 0.081 2007 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Bogaert, Yolanda E. Levi, Moshe Sorribas, Víctor Universidad de Zaragoza (orcid)0000-0003-3457-323X 1000 807 Universidad de Zaragoza Dpto. Anat.Pat.Med.Leg.For.To. Area Toxicología 27, 5 (2007), 1030-1036 Arterioscler. thromb. vasc. biol. Arteriosclerosis, Thrombosis, and Vascular Biology 1079-5642 403767 http://zaguan.unizar.es/record/165691/files/texto_completo.pdf Postprint 919715 http://zaguan.unizar.es/record/165691/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:165691 articulos driver 2026-01-13-22:06:03 ARTICLE