000165708 001__ 165708
000165708 005__ 20260113234335.0
000165708 0247_ $$2doi$$a10.3390/ani13061043
000165708 0248_ $$2sideral$$a133023
000165708 037__ $$aART-2023-133023
000165708 041__ $$aeng
000165708 100__ $$0(orcid)0000-0002-6806-9990$$aHernaiz, Adelaida$$uUniversidad de Zaragoza
000165708 245__ $$aSusceptibility of ovine bone marrow-derived mesenchymal stem cell spheroids to scrapie prion infection
000165708 260__ $$c2023
000165708 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165708 5203_ $$aIn neurodegenerative diseases, including prion diseases, cellular in vitro models appear as fundamental tools for the study of pathogenic mechanisms and potential therapeutic compounds. Two-dimensional (2D) monolayer cell culture systems are the most used cell-based assays, but these platforms are not able to reproduce the microenvironment of in vivo cells. This limitation can be surpassed using three-dimensional (3D) culture systems such as spheroids that more effectively mimic in vivo cell interactions. Herein, we evaluated the effect of scrapie prion infection in monolayer-cultured ovine bone marrow-derived mesenchymal stem cells (oBM-MSCs) and oBM-MSC-derived spheroids in growth and neurogenic conditions, analyzing their cell viability and their ability to maintain prion infection. An MTT assay was performed in oBM-MSCs and spheroids subjected to three conditions: inoculated with brain homogenate from scrapie-infected sheep, inoculated with brain homogenate from healthy sheep, and non-inoculated controls. The 3D conditions improved the cell viability in most cases, although in scrapie-infected spheroids in growth conditions, a decrease in cell viability was observed. The levels of pathological prion protein (PrPSc) in scrapie-infected oBM-MSCs and spheroids were measured by ELISA. In neurogenic conditions, monolayer cells and spheroids maintained the levels of PrPSc over time. In growth conditions, however, oBM-MSCs showed decreasing levels of PrPSc throughout time, whereas spheroids were able to maintain stable PrPSc levels. The presence of PrPSc in spheroids was also confirmed by immunocytochemistry. Altogether, these results show that a 3D culture microenvironment improves the permissiveness of oBM-MSCs to scrapie infection in growth conditions and maintains the infection ability in neurogenic conditions, making this model of potential use for prion studies.
000165708 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A19-20R$$9info:eu-repo/grantAgreement/ES/DGA-FSE/IIU-2023-2017$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00
000165708 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000165708 590__ $$a2.7$$b2023
000165708 591__ $$aVETERINARY SCIENCES$$b16 / 167 = 0.096$$c2023$$dQ1$$eT1
000165708 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b10 / 80 = 0.125$$c2023$$dQ1$$eT1
000165708 592__ $$a0.698$$b2023
000165708 593__ $$aVeterinary (miscellaneous)$$c2023$$dQ1
000165708 593__ $$aAnimal Science and Zoology$$c2023$$dQ1
000165708 594__ $$a4.9$$b2023
000165708 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165708 700__ $$aCobeta, Paula
000165708 700__ $$0(orcid)0000-0002-1590-3347$$aMarín, Belén$$uUniversidad de Zaragoza
000165708 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, Francisco José$$uUniversidad de Zaragoza
000165708 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan José$$uUniversidad de Zaragoza
000165708 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000165708 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000165708 700__ $$aMartín-Burriel, Inmaculada
000165708 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000165708 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000165708 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000165708 773__ $$g13, 6 (2023), 1043 [18 pp.]$$pAnimals (Basel)$$tAnimals$$x2076-2615
000165708 8564_ $$s6295757$$uhttps://zaguan.unizar.es/record/165708/files/texto_completo.pdf$$yVersión publicada
000165708 8564_ $$s2684714$$uhttps://zaguan.unizar.es/record/165708/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165708 909CO $$ooai:zaguan.unizar.es:165708$$particulos$$pdriver
000165708 951__ $$a2026-01-13-22:06:30
000165708 980__ $$aARTICLE