000165750 001__ 165750 000165750 005__ 20260113234335.0 000165750 0247_ $$2doi$$a10.1016/j.taap.2010.05.012 000165750 0248_ $$2sideral$$a72452 000165750 037__ $$aART-2010-72452 000165750 041__ $$aeng 000165750 100__ $$aVilla-Bellosta, R.$$uUniversidad de Zaragoza 000165750 245__ $$aArsenate transport by sodium/phosphate cotransporter type IIb 000165750 260__ $$c2010 000165750 5060_ $$aAccess copy available to the general public$$fUnrestricted 000165750 5203_ $$aArsenic is a metalloid that causes the dysfunction of critical enzymes, oxidative stress, and malignancies. In recent years several transporters of AsIII have been identified, including aquaglyceroporins (AQP) and multidrug resistance proteins (MRP). AsV transport, however, has not been sufficiently studied because it has been assumed that arsenate is taken up by mammalian cells through inorganic phosphate (Pi) transporters. In this paper we have analyzed the role of Pi transporters in the uptake of arsenate by directly using 73AsV as a radiotracer in phosphate transporter-expressing Xenopus laevis oocytes. The affinities of Pi transporters for H3AsO4 were lower than the affinities for Pi. NaPiIIa, NaPiIIc, Pit1, and Pit2 showed a Km for arsenate that was > 1 mM (i.e., at least ten times lower than the affinities for Pi). The NaPiIIb isoform showed the highest affinity for AsV in mouse (57 μM), rat (51 μM), and human (9.7 μM), which are very similar to the affinities for Pi. Therefore, NaPiIIb can have a prominent role in the toxicokinetics of arsenic following oral exposure to freshwater or food contaminated with AsV. 000165750 536__ $$9info:eu-repo/grantAgreement/ES/MEC/BFU2016-06284-BFI 000165750 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 000165750 590__ $$a3.993$$b2010 000165750 591__ $$aTOXICOLOGY$$b10 / 83 = 0.12$$c2010$$dQ1$$eT1 000165750 591__ $$aPHARMACOLOGY & PHARMACY$$b41 / 252 = 0.163$$c2010$$dQ1$$eT1 000165750 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000165750 700__ $$0(orcid)0000-0003-3457-323X$$aSorribas, V.$$uUniversidad de Zaragoza 000165750 7102_ $$11000$$2807$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Toxicología 000165750 773__ $$g247, 1 (2010), 36-40$$pToxicol. appl. pharmacol.$$tTOXICOLOGY AND APPLIED PHARMACOLOGY$$x0041-008X 000165750 8564_ $$s239018$$uhttps://zaguan.unizar.es/record/165750/files/texto_completo.pdf$$yPostprint 000165750 8564_ $$s529484$$uhttps://zaguan.unizar.es/record/165750/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000165750 909CO $$ooai:zaguan.unizar.es:165750$$particulos$$pdriver 000165750 951__ $$a2026-01-13-22:07:33 000165750 980__ $$aARTICLE