000165892 001__ 165892
000165892 005__ 20260115140311.0
000165892 0247_ $$2doi$$a10.1021/jacs.5c16679
000165892 0248_ $$2sideral$$a147424
000165892 037__ $$aART-2026-147424
000165892 041__ $$aeng
000165892 100__ $$aVera-Arévalo, Mauricio$$uUniversidad de Zaragoza
000165892 245__ $$aFluorescence Transduction of Liquid Crystal Ordering Transitions for Biosensing
000165892 260__ $$c2026
000165892 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165892 5203_ $$aLiquid crystal (LC) ordering transitions are exquisitely sensitive to molecular interactions at aqueous interfaces and have long served as the basis for optical biosensors. However, the readout of these transitions has almost exclusively relied on polarized-light optical microscopy, which limits quantification and hinders practical deployment. Here, we report a fluorescence-based transduction scheme that converts LC ordering transitions to quantitative optical outputs. Our strategy employs amphiphilic block copolymers bearing aggregation-induced emission (AIE) motifs that undergo dynamic covalent conjugation with IgG antibodies through reversible imine chemistry. In complex LC emulsions, polymer surfactants localize differently depending on droplet LC configuration: accumulation at monopolar defects concentrates AIE units to generate a bright ON state, whereas redistribution along the LC/water interface in the radial configuration suppresses emission to yield an OFF state. Recognition of Salmonella enterica serovar Typhimurium─one of the most prevalent foodborne pathogens─reversibly perturbs this equilibrium, producing rapid (∼1 h) ON/OFF fluorescence responses with detection limits down to 102 cells/mL. Incorporation of a ratiometric reference dye further enhances robustness against experimental variability. This work establishes the fluorescence transduction of LC ordering transitions as a generalizable and portable sensing paradigm, bridging soft matter design with real-world diagnostics.
000165892 536__ $$9info:eu-repo/grantAgreement/ES/MICIU CEX2023-001286-S
000165892 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000165892 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165892 700__ $$0(orcid)0000-0002-8932-9085$$aConcellón, Alberto$$uUniversidad de Zaragoza
000165892 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000165892 773__ $$pJ. Am. Chem. Soc.$$tJournal of the American Chemical Society$$x0002-7863
000165892 8564_ $$s7604224$$uhttps://zaguan.unizar.es/record/165892/files/texto_completo.pdf$$yVersión publicada
000165892 8564_ $$s3221485$$uhttps://zaguan.unizar.es/record/165892/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165892 909CO $$ooai:zaguan.unizar.es:165892$$particulos$$pdriver
000165892 951__ $$a2026-01-15-12:37:25
000165892 980__ $$aARTICLE