165902 20260116002211.0 doi 10.1111/cmi.12169 sideral 84683 ART-2013-84683 eng Aguiló, JI Universidad de Zaragoza (orcid)0000-0001-7897-9173 ESX-1-induced apoptosis is involved in cell-to-cell spread of Mycobacterium tuberculosis. 2013 Apoptosis modulation is a procedure amply utilized by intracellular pathogens to favour the outcome of the infection. Nevertheless, the role of apoptosis during infection with Mycobacterium tuberculosis, the causative agent of human tuberculosis, is subject of an intense debate and still remains unclear. In this work, we describe that apoptosis induction in host cells is clearly restricted to virulent M. tuberculosis strains, and is associated with the capacity of the mycobacteria to secrete the 6 kDa early secreted antigenic target ESAT-6 both under in vitro and in vivo conditions. Remarkably, only apoptosis-inducing strains are able to propagate infection into new cells, suggesting that apoptosis is used by M. tuberculosis as a colonization mechanism. Finally, we demonstrate that in vitro modulation of apoptosis affects mycobacterial cell-to-cell spread capacity, establishing an unambiguous relationship between apoptosis and propagation of M. tuberculosis. Our data further indicate that BCG and MTBVAC vaccines are inefficient in inducing apoptosis and colonizing new cells, correlating with the strong attenuation profile of these strains previously observed in vitro and in vivo. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.816 2013 MICROBIOLOGY 20 / 118 = 0.169 2013 Q1 T1 CELL BIOLOGY 55 / 184 = 0.299 2013 Q2 T1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Alonso, H Universidad de Zaragoza (orcid)0000-0003-2742-8827 Uranga, S Universidad de Zaragoza (orcid)0000-0001-7866-2803 Marinova, D Universidad de Zaragoza (orcid)0000-0001-8644-120X Arbués, A (orcid)0000-0002-3377-4171 de Martino, A Anel, A Universidad de Zaragoza (orcid)0000-0002-5175-8394 Monzon, M Badiola, J Universidad de Zaragoza (orcid)0000-0002-7173-7216 Pardo, J Universidad de Zaragoza (orcid)0000-0003-0154-0730 Brosch, R Martin, C Universidad de Zaragoza (orcid)0000-0003-2993-5478 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1008 566 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Inmunología 1008 X Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Proy. investigación HQA 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 15, 12 (2013), 1994-2005 Cell. microbiol. CELLULAR MICROBIOLOGY 1462-5814 1195135 http://zaguan.unizar.es/record/165902/files/texto_completo.pdf Postprint 1127799 http://zaguan.unizar.es/record/165902/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:165902 articulos driver 2026-01-15-21:56:12 ARTICLE