000165992 001__ 165992
000165992 005__ 20260116163026.0
000165992 0247_ $$2doi$$a10.1016/j.fm.2025.105014
000165992 0248_ $$2sideral$$a147466
000165992 037__ $$aART-2026-147466
000165992 041__ $$aeng
000165992 100__ $$aLytras, Fotios
000165992 245__ $$aInsights on the microbial resistance mechanisms of listeria monocytogenes to Pulsed Electric Fields (PEF) treatments
000165992 260__ $$c2026
000165992 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165992 5203_ $$aThis study aimed to identify the principal mechanisms of action by which Listeria monocytogenes EGD-e responds to pulsed electric field (PEF) treatments at pH 7.0, given its recognition as a robust target microorganism and strain. Microbiologically challenged buffer samples (pH 7.0) were subjected to pulses with an electric field strength of 20 kV/cm and their transcriptional response was assessed using RNA sequencing. Our analysis revealed 119 differentially expressed genes, 51 of which were upregulated and 68 downregulated. From the 51 upregulated genes, 4 were transcription regulators (lmo1974, glnR, lmo806 and lmo0371) with the potential to influence the resistance of L. monocytogenes EGD-e. Additionally, assessment of 11 isogenic mutants at a PEF treatment (20 kV/cm, 184 kJ/kg) relative to the wild type identified the ΔyneA and ΔclpB deletion mutants as more resistant and more sensitive (p<0.05). Finally, the isogenic mutant ΔclpB was assessed against the wild type at 25 kV/cm at different total specific energies (54, 113, 135 and 160 kJ/kg) resulting in statistical difference(p<0.05) only under the highest parameter. In conclusion, transcriptomic analysis revealed that the primary mechanistic pathways of L. monocytogenes in response to PEF involve the preservation of homeostasis, energy availability, and quorum sensing. Additionally, the increased sensitivity of the ΔclpB mutant highlights a supplementary mechanism related to protein disaggregation and refolding under high-energy. These findings suggest that L. monocytogenes mounts a complex and multifaceted response to PEF treatments. These results can provide insights and support PEF treatment decontamination alone or as pretreatment in combination with other hurdles.
000165992 536__ $$9info:eu-repo/grantAgreement/EC/H2020/955431/EU/Training Network Sustainable Technologies/TRANSIT$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 955431-TRANSIT
000165992 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttps://creativecommons.org/licenses/by-nc/4.0/deed.es
000165992 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165992 700__ $$aIhsan, Muhammad Ahmed
000165992 700__ $$aPsakis, Georgios
000165992 700__ $$aGatt, Ruben
000165992 700__ $$0(orcid)0000-0002-5049-3646$$aCebrián, Guillermo$$uUniversidad de Zaragoza
000165992 700__ $$0(orcid)0000-0003-3957-9091$$aRaso, Javier$$uUniversidad de Zaragoza
000165992 700__ $$aValdramidis, Vasilis P.
000165992 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000165992 773__ $$g136 (2026), 105014 [11 pp.]$$pFood microbiol.$$tFOOD MICROBIOLOGY$$x0740-0020
000165992 8564_ $$s1723035$$uhttps://zaguan.unizar.es/record/165992/files/texto_completo.pdf$$yVersión publicada
000165992 8564_ $$s2586511$$uhttps://zaguan.unizar.es/record/165992/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165992 909CO $$ooai:zaguan.unizar.es:165992$$particulos$$pdriver
000165992 951__ $$a2026-01-16-14:54:25
000165992 980__ $$aARTICLE