166090 20260119170326.0 doi 10.1007/s12094-025-03854-9 sideral 147504 ART-2025-147504 eng Galindo Torres, Blanca Pilar New evidence for miRNA testing in head and neck squamous cell cancer patients 2025 Purpose: Prognosis of HNSCC has not changed over the last decades. MicroRNAs mediate gene expression and participate in regulating cellular biological processes. Its aberrant expression is an important event in the development of several cancers, including head and neck squamous cell cancer. The aim of the study is to determine if circulating miRNAs are reliable diagnostic indicators and can be used to monitor head and neck cancer. Methods/Patients: An observational, longitudinal, prospective, analytical study was conducted, with a case-control design, in which 37 head and neck squamous cell cancer patients at diagnosis were compared with 30 healthy patients. Blood samples were obtained and free miRNA expression levels of 17 miRNAs were determined by PCR-RT. Follow-up of HNSCC was carried out for one year with blood extractions at 7 days for surgical patients, and 1, 2, 6 and 12 months after finishing treatment for all patients. Results: Seventy-eight percent of the participants in HNSCC group and 57% among control group were men. Smokers and alcohol consumers exhibit increased susceptibility to HNSCC, and risk rises to 63,4% (R2 = 0,634) when both factors are combined. HNSCC patients overexpressed miR-21-5p and miR-122, while miR-195-5p is downregulated. Elevated miR-21-5p levels correlates with tumour size and miR- 374b-5p, with advanced stage (p=0.005). Conclusion: Our findings suggest that the evaluation of certain miRNAs’ expression levels in plasma can be used as potential markers for HNSCC diagnosis. Further assays with larger samples could be performed to validate data and establish a cut-off Access copy available to the general public Unrestricted info:eu-repo/semantics/embargoedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Alcaraz Ortega, Raquel Saiz López, Patricia Adiego Leza, María Isabel Universidad de Zaragoza (orcid)0000-0002-3265-504X Moradillo Renuncio, María del Mar García Girón, Carlos Grijalba Uche, Miguel Víctor 1013 653 Universidad de Zaragoza Dpto. Cirugía Área Otorrinolaringología 27, 8 (2025), 3399-3409 Clin. transl. oncol. Clinical and Translational Oncology 1699-048X 1577070 http://zaguan.unizar.es/record/166090/files/texto_completo.pdf Postprint info:eu-repo/date/embargoEnd/2026-02-06 2305348 http://zaguan.unizar.es/record/166090/files/texto_completo.jpg?subformat=icon icon Postprint info:eu-repo/date/embargoEnd/2026-02-06 oai:zaguan.unizar.es:166090 articulos driver 2026-01-19-14:39:47 ARTICLE