000167854 001__ 167854
000167854 005__ 20260121151419.0
000167854 0247_ $$2doi$$a10.1099/mgen.0.001581
000167854 0248_ $$2sideral$$a147537
000167854 037__ $$aART-2025-147537
000167854 041__ $$aeng
000167854 100__ $$aBenamrouche, Nabila
000167854 245__ $$aGenomic characterisation of multidrug-resistant Salmonella enterica serovar Kentucky ST198 isolates from various sources in Algeria, North Africa
000167854 260__ $$c2025
000167854 5060_ $$aAccess copy available to the general public$$fUnrestricted
000167854 5203_ $$aSalmonella enterica serovar Kentucky (S. Kentucky) sequence type (ST) 198 has emerged as a globally disseminated multidrugresistant (MDR) lineage posing significant public health challenges. The aim of this study was to characterise 125S. Kentucky ST198 isolates collected from various sources in Algeria, including humans, animals, food and the environment, or obtained from humans in France (travellers returning from Algeria), between 2015 and 2022. Whole-genome sequencing was performed on 125 isolates to assess their genetic diversity and antimicrobial resistance (AMR) profiles. Phylogenetic analysis revealed that the Algerian S. Kentucky ST198 isolates were closely related to each other and belonged to the MDR lineage that emerged in Egypt before disseminating into Africa, the Middle East, Asia and Europe. These isolates also clustered closely with European and North African isolates carrying the gyrA_D87N mutation. We found that 90% of the isolates had MDR phenotypes,
with resistance to critically important antibiotics, including ciprofloxacin, third-generation cephalosporins, azithromycin and chloramphenicol. Genomic analysis revealed that all the isolates had the three known non-synonymous resistance mutations in the quinolone-resistance-determining regions of DNA gyrase (gyrA) and DNA topoisomerase IV (parC). Multiple AMR genes were also identified, including blaCTX-M-15, blaCMY-2, blaCMY-4, qnrB19, mph(A), cmlA1 and floR. The Algerian isolates also contained the main variant of SGI1, SGI1-K, with multiple rearrangements. Plasmid replicon analysis revealed that the most frequent plasmid types were IncI1 (13.6%), Col156 (8.8%) and Col(pVC) (8%). This study provides the first comprehensive genomic insight into S. Kentucky ST198 in Algeria, highlighting the urgent need for a reinforcement of AMR surveillance and control measures in the region. These findings enhance our overall understanding of the epidemiology and evolution of MDR Salmonella and highlight
the importance of a One Health approach for combating the spread of resistant pathogens.
000167854 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000167854 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000167854 700__ $$aBelkader, Chafika
000167854 700__ $$aDeriet-Ammar, Abdehamid
000167854 700__ $$0(orcid)0000-0002-3273-6392$$aPardos de la Gandara, Maria$$uUniversidad de Zaragoza
000167854 700__ $$aSihem Zemam, Sarah
000167854 700__ $$aNjamkepo, Elisabeth
000167854 700__ $$aSadat, Soraya
000167854 700__ $$aMsela, Amine
000167854 700__ $$aFabre, Laëtitia
000167854 700__ $$aMechouet, Faiza
000167854 700__ $$aTorkya Boutabba, Dalila
000167854 700__ $$aSlimani, Rym
000167854 700__ $$aWeill, François-Xavier
000167854 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000167854 773__ $$g11, 11 (2025), 001581[11 pp.]$$pMicrob. genom.$$tMicrobial genomics$$x2057-5858
000167854 8564_ $$s2405243$$uhttps://zaguan.unizar.es/record/167854/files/texto_completo.pdf$$yVersión publicada
000167854 8564_ $$s2562718$$uhttps://zaguan.unizar.es/record/167854/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000167854 909CO $$ooai:zaguan.unizar.es:167854$$particulos$$pdriver
000167854 951__ $$a2026-01-21-14:54:56
000167854 980__ $$aARTICLE