000167892 001__ 167892
000167892 005__ 20260122111948.0
000167892 0247_ $$2doi$$a10.1007/s10719-025-10202-0
000167892 0248_ $$2sideral$$a147336
000167892 037__ $$aART-2025-147336
000167892 041__ $$aeng
000167892 100__ $$aHu, Zi-Xuan
000167892 245__ $$aIdentification of a distinct sialic acid (KDN) and a KDN-specific aldolase in Pacific oyster
000167892 260__ $$c2025
000167892 5203_ $$aSialic acids are a diverse family of acidic sugars typically found at the terminal positions of glycan chains, mediating key physiological and pathological processes across animals - particularly vertebrates - including cell signaling and host-pathogen interactions. The distribution of sialic acids in lower animals such as mollusks, however, remains largely unresolved. Here, we report the discovery of unconjugated 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), a deaminated
analogue of N-acetylneuraminic acid, in the muscle tissue of Pacific oysters (Magallana gigas). Using UPLC-ESI-MS/MS fingerprinting, we identified naturally occurring free KDN at a concentration of 1.2 ± 0.1 nmol/100 mg of oyster muscle
tissue. To investigate the biosynthetic pathway, four candidate genes were identified in the M. gigas genome, and the corresponding recombinant proteins were expressed and characterized. Enzymatic assays revealed that one putative sialic
acid aldolase (MgNPL) specifically catalyzes the cleavage of KDN into mannose and pyruvate. To our knowledge, this represents the first molecular evidence of KDN metabolism in mollusks and highlights both the unexpected conservation of substrate-specific aldolase activity and distinct sialic acid utilization mechanisms compared to vertebrates.
000167892 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000167892 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000167892 700__ $$aZhang, Jia-Yu
000167892 700__ $$avan Ede, Jitske
000167892 700__ $$aZhang, Yao-Yao
000167892 700__ $$aLi, Yu-Quan
000167892 700__ $$0(orcid)0000-0002-2855-4801$$aGhirardello, Mattia$$uUniversidad de Zaragoza
000167892 700__ $$aGalan, M. Carmen
000167892 700__ $$aPabst, Martin
000167892 700__ $$aLiu, Li
000167892 700__ $$aVoglmeir, Josef
000167892 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000167892 773__ $$g43, 1 (2025), [10 pp.]$$pGlycoconj. j.$$tGLYCOCONJUGATE JOURNAL$$x0282-0080
000167892 8564_ $$s1584573$$uhttps://zaguan.unizar.es/record/167892/files/texto_completo.pdf$$yVersión publicada$$zinfo:eu-repo/date/embargoEnd/2026-12-19
000167892 8564_ $$s2246366$$uhttps://zaguan.unizar.es/record/167892/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada$$zinfo:eu-repo/date/embargoEnd/2026-12-19
000167892 909CO $$ooai:zaguan.unizar.es:167892$$particulos$$pdriver
000167892 951__ $$a2026-01-22-10:26:55
000167892 980__ $$aARTICLE