167979 20260128112902.0 doi 10.1016/j.bbabio.2008.12.006 sideral 61050 ART-2009-61050 eng Goñí, Guillermina (orcid)0000-0003-4010-849X Flavodoxin: A compromise between efficiency and versatility in the electron transfer from Photosystem I to Ferredoxin-NADP(+) reductase. 2009 Access copy available to the general public Unrestricted Under iron-deficient conditions Flavodoxin (Fld) replaces Ferredoxin in Anabaena as electron carrier from Photosystem I (PSI) to Ferredoxin-NADP+ reductase (FNR). Several residues modulate the Fld interaction with FNR and PSI, but no one appears as specifically critical for efficient electron transfer (ET). Fld shows a strong dipole moment, with its negative end directed towards the flavin ring. The role of this dipole moment in the processes of interaction and ET with positively charged surfaces exhibited by PSI and FNR has been analysed by introducing single and multiple charge reversal mutations on the Fld surface. Our data confirm that in this system interactions do not rely on a precise complementary surface of the reacting molecules. In fact, they indicate that the initial orientation driven by the alignment of dipole moment of the Fld molecule with that of the partner contributes to the formation of a bunch of alternative binding modes competent for the efficient ET reaction. Additionally, the fact that Fld uses different interaction surfaces to dock to PSI and to FNR is confirmed. info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 3.688 2009 BIOPHYSICS 18 / 74 = 0.243 2009 Q1 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 87 / 281 = 0.31 2009 Q2 T1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Herguedas, Beatriz Universidad de Zaragoza (orcid)0000-0003-2044-4795 Hervás, Manuel Peregrina, José R. de la Rosa, Miguel A. Gómez-Moreno, Carlos Universidad de Zaragoza (orcid)0000-0003-4087-4733 Navarro, José A. Hermoso, Juan A. Martínez-Júlvez, Marta Universidad de Zaragoza (orcid)0000-0001-9047-0046 Medina, Milagros Universidad de Zaragoza (orcid)0000-0001-8743-0182 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1787, 3 (2009), 144-154 Biochim. biophys. acta, Bioenerg. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 0005-2728 6042632 http://zaguan.unizar.es/record/167979/files/texto_completo.pdf Postprint 1252549 http://zaguan.unizar.es/record/167979/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:167979 articulos driver 2026-01-28-11:24:54 ARTICLE