000167989 001__ 167989
000167989 005__ 20260123152958.0
000167989 0247_ $$2doi$$a10.1038/sj.ki.5002703
000167989 0248_ $$2sideral$$a66552
000167989 037__ $$aART-2008-66552
000167989 041__ $$aeng
000167989 100__ $$aVilla-Bellosta, R.
000167989 245__ $$aInteractions of the Growth-Related, Type IIc Renal sodium/phosphate Cotransporter with PDZ Proteins
000167989 260__ $$c2008
000167989 5060_ $$aAccess copy available to the general public$$fUnrestricted
000167989 5203_ $$aDespite similar molecular structures, the growth-related sodium/phosphate cotransporter NaPiIIc is regulated differently than the main NaPiIIa phosphate transporter. Using two-hybrid systems and immunoprecipitation, we identified several proteins that interact with NaPiIIc that might account for this differential regulation. NaPiIIc interacted with the PDZ domain-containing sodium–hydrogen exchange-regulating factor (NHERF) 1 and NHERF3 through novel binding motifs in its C terminus. NaPiIIc from brush-border membranes coprecipitated with both NHERF1 and NHERF3, with more NHERF3 co-precipitated in rats fed a low-phosphorus diet. NaPiIIc colocalizes with both NHERF1 and NHERF3 in brush-border membranes of rats fed either a low- or high-phosphorus diet. When mouse NaPiIIc was transfected into opossum kidney cells, it was localized mainly in apical microvilli and the trans-Golgi. Both confocal and total internal reflection microscopy show that NaPiIIc colocalizes with NHERF1 and NHERF3 in the apical microvilli, and this was not altered by truncation of the last three amino acids of NaPiIIc. Interactions of NaPiIIc with NHERF1 and NHERF3 were modulated by the membrane-associated 17 kDa protein (MAP17) similarly to NaPiIIa, but only the MAP17–NaPiIIc–NHERF3 complexes were internalized to the trans-Golgi. Our study shows that NaPiIIc interacts with a limited number of PDZ domain proteins, and the mechanisms and consequences of such interactions differ from those of NaPiIIa.
000167989 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000167989 590__ $$a6.418$$b2008
000167989 591__ $$aUROLOGY & NEPHROLOGY$$b3 / 57 = 0.053$$c2008$$dQ1$$eT1
000167989 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000167989 700__ $$aBarac-Nieto,M.
000167989 700__ $$aBreusegem, S.Y.
000167989 700__ $$aBarry, N.P.
000167989 700__ $$aLevi, M.
000167989 700__ $$0(orcid)0000-0003-3457-323X$$aSorribas, V.$$uUniversidad de Zaragoza
000167989 7102_ $$11000$$2807$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Toxicología
000167989 773__ $$g73, 4 (2008), 456-464$$pKidney int.$$tKIDNEY INTERNATIONAL$$x0085-2538
000167989 8564_ $$s2417613$$uhttps://zaguan.unizar.es/record/167989/files/texto_completo.pdf$$yPostprint
000167989 8564_ $$s2183963$$uhttps://zaguan.unizar.es/record/167989/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000167989 909CO $$ooai:zaguan.unizar.es:167989$$particulos$$pdriver
000167989 951__ $$a2026-01-23-14:32:55
000167989 980__ $$aARTICLE