000168030 001__ 168030
000168030 005__ 20260123152959.0
000168030 0247_ $$2doi$$a10.1186/s13071-025-07130-1
000168030 0248_ $$2sideral$$a147638
000168030 037__ $$aART-2025-147638
000168030 041__ $$aeng
000168030 100__ $$aPichler, Verena
000168030 245__ $$aTracking pyrethroid resistance in arbovirus mosquito vectors: mutations I1532T and F1534C in Aedes albopictus across Europe
000168030 260__ $$c2025
000168030 5060_ $$aAccess copy available to the general public$$fUnrestricted
000168030 5203_ $$aBackground With the worldwide spread of the Asian tiger mosquito, Aedes albopictus, the number of autochthonous cases of exotic arboviral diseases, such as dengue or chikungunya, is increasing in temperate regions. In Europe, pyrethroids are the only insecticides allowed for the abatement of adult mosquitoes and are thus crucial for limiting ongoing arbovirus transmission. Despite this and the report of resistance rising in vector populations worldwide, information on the pyrethroid resistance status of vector populations and knowledge on resistance mechanisms is widely lacking. Genotyping of knockdown resistance (kdr) mutations situated within the target site of pyrethroids, i.e., the voltage-gated sodium channel (VGSC), and associated with pyrethroid resistance, is a cost-effective approach to investigate the spread of resistance in a population. Herein, we describe the European-wide distribution of two kdr mutations, i.e., I1532T and F1534C, in Ae. albopictus and evaluate their co-occurrence with another well-characterized kdr mutation, V1016G. Methods Genotyping of the kdr mutation F1534C was performed by allele-specific PCR for 1732 Ae. albopictus specimens sampled in 19 European countries; for a subset of 419 specimens mutation I1532T was also genotyped by sequencing. For all samples, information on mutation V1016G was available, allowing evaluation of the co-occurrence of kdr alleles. Results Mutation 1534C was detected in nine sites from six countries at an overall frequency close to 5%. Highest frequencies per site were detected in Cyprus (84%) and Greece (45%). Allele 1532 T was identified in 11 sites from 7 countries at frequencies ranging from 4% to 25% per site. Co-occurrence of different kdr alleles (1534C, 1532 T and 1016G) was observed in nine sampling sites from seven countries. Conclusions The present study offers the first map of the occurrence of the major Ae. albopictus kdr alleles across Europe and highlights a differential distribution of the two alleles most strongly associated with pyrethroid resistance, 1016G and 1534C. Our findings also point to the need for enhancing resistance monitoring in the Eastern Mediterranean region, where the two mutations are shown to exist in geographically close areas, with the risk of emergence of highly resistant double mutants.
000168030 536__ $$9info:eu-repo/grantAgreement/EUR/COST-Action/CA17108
000168030 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000168030 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000168030 700__ $$aValadas, Vera
000168030 700__ $$aAkiner, Mustafa M.
000168030 700__ $$aBalatsos, Georgios
000168030 700__ $$aBarceló, Carlos
000168030 700__ $$aBorg, Maria Louise
000168030 700__ $$aBouyer, Jeremy
000168030 700__ $$aBravo-Barriga, Daniel
000168030 700__ $$aBueno, Ruben
000168030 700__ $$aCaputo, Beniamino
000168030 700__ $$aCollantes, Francisco
000168030 700__ $$0(orcid)0000-0001-7046-2997$$aDelacour-Estrella, Sarah$$uUniversidad de Zaragoza
000168030 700__ $$aVelo, Enkelejda
000168030 700__ $$aFalcuta, Elena
000168030 700__ $$aFlacio, Eleonora
000168030 700__ $$aGarcía-Pérez, Ana L.
000168030 700__ $$aGómez, José F.
000168030 700__ $$aHorvath, Cintia
000168030 700__ $$aAdam, Katja
000168030 700__ $$aKadriaj, Perparim
000168030 700__ $$aKavran, Mihaela
000168030 700__ $$aL’Ambert, Gregory
000168030 700__ $$aLia, Riccardo P.
000168030 700__ $$aMarabuto, Eduardo
000168030 700__ $$aMedialdea-Carrera, Raquel
000168030 700__ $$aMelero-Alcibar, Rosario
000168030 700__ $$aMichaelakis, Antonios
000168030 700__ $$aMihalca, Andrei Daniel
000168030 700__ $$aMicocci, Martina
000168030 700__ $$aMikov, Ognyan
000168030 700__ $$aMiranda, Miguel A.
000168030 700__ $$aMüller, Pie
000168030 700__ $$aOrnosa, Concepción
000168030 700__ $$aOuterelo, Raimundo
000168030 700__ $$aOtranto, Domenico
000168030 700__ $$aPajovic, Igor
000168030 700__ $$aPérez-Tris, Javier
000168030 700__ $$aPetric, Dusan
000168030 700__ $$aRebelo, Maria Teresa
000168030 700__ $$aBesnard, Gilles
000168030 700__ $$aRogozi, Elton
000168030 700__ $$aTello, Ana
000168030 700__ $$aVázquez, Ángeles
000168030 700__ $$aVasquez, Marlen
000168030 700__ $$aZitko, Toni
000168030 700__ $$aSchaffner, Francis
000168030 700__ $$adella Torre, Alessandra
000168030 700__ $$aPinto, Joao
000168030 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000168030 773__ $$g18 (2025), 506 [11 pp.]$$pParasit. vectors$$tParasites & vectors$$x1756-3305
000168030 8564_ $$s1743289$$uhttps://zaguan.unizar.es/record/168030/files/texto_completo.pdf$$yVersión publicada
000168030 8564_ $$s2257450$$uhttps://zaguan.unizar.es/record/168030/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000168030 909CO $$ooai:zaguan.unizar.es:168030$$particulos$$pdriver
000168030 951__ $$a2026-01-23-14:33:43
000168030 980__ $$aARTICLE