000168033 001__ 168033
000168033 005__ 20260123152959.0
000168033 0247_ $$2doi$$a10.1038/s41467-025-66871-w
000168033 0248_ $$2sideral$$a147636
000168033 037__ $$aART-2025-147636
000168033 041__ $$aeng
000168033 100__ $$0(orcid)0000-0002-2855-4801$$aGhirardello, Mattia$$uUniversidad de Zaragoza
000168033 245__ $$aStructure, function, and implications of fucosyltransferases in health and disease
000168033 260__ $$c2025
000168033 5060_ $$aAccess copy available to the general public$$fUnrestricted
000168033 5203_ $$aFucosylation is a ubiquitous glycosylation event that shapes cellular communication and immunity. Catalyzed by fucosyltransferases (FUTs), this reaction encompasses diverse substrates, mechanisms, and biologic consequences. In this Review, we explore the structural and functional landscape of FUTs primarily from higher eukaryotes, with focus on the mechanistic determinants of regioselectivity, donor/acceptor coordination, and domain modularity. We highlight advances in structural biology, modeling, and enzyme engineering that clarify how FUTs decode glycan topology and specificity. Phylogenetic and structural analyses reveal two major clades of human FUTs that differ in GDP-Fuc recognition and conformational flexibility, providing a molecular rationale for their mechanistic divergence. Drawing from mammalian FUT studies, we propose a conceptual framework in which distinct family members exploit strategies including donor-induced conformational changes, exosite interactions, or local peptide cues to achieve specificity and catalytic efficiency. We also examine their roles in physiology, inflammation, immune regulation, and cancer, and summarize current FUT inhibitors and enzymebased therapeutic strategies.
000168033 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E34-20R$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-23R$$9info:eu-repo/grantAgreement/ES/MICINN AEI/PID2022-136362NB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-137973NB-I00
000168033 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000168033 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000168033 700__ $$0(orcid)0000-0003-3608-4720$$aYruela, Inmaculada
000168033 700__ $$0(orcid)0000-0002-2202-3460$$aMerino, Pedro$$uUniversidad de Zaragoza
000168033 700__ $$aSackstein, Robert
000168033 700__ $$aSanz-Martínez, Ignacio$$uUniversidad de Zaragoza
000168033 700__ $$0(orcid)0000-0002-3122-9401$$aHurtado-Guerrero, Ramón
000168033 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000168033 773__ $$g16 (2025), 11279 [28 pp.]$$tNature communications$$x2041-1723
000168033 8564_ $$s4505321$$uhttps://zaguan.unizar.es/record/168033/files/texto_completo.pdf$$yVersión publicada
000168033 8564_ $$s2616697$$uhttps://zaguan.unizar.es/record/168033/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000168033 909CO $$ooai:zaguan.unizar.es:168033$$particulos$$pdriver
000168033 951__ $$a2026-01-23-14:33:47
000168033 980__ $$aARTICLE