000168150 001__ 168150
000168150 005__ 20260127160850.0
000168150 0247_ $$2doi$$a10.1002/anie.201609758
000168150 0248_ $$2sideral$$a147685
000168150 037__ $$aART-2017-147685
000168150 041__ $$aeng
000168150 100__ $$0(orcid)0000-0001-9478-6750$$aVelasco-Lozano, Susana
000168150 245__ $$aCo‐immobilized Phosphorylated Cofactors and Enzymes as Self‐Sufficient Heterogeneous Biocatalysts for Chemical Processes
000168150 260__ $$c2017
000168150 5060_ $$aAccess copy available to the general public$$fUnrestricted
000168150 5203_ $$aEnzyme cofactors play a major role in biocatalysis, as many enzymes require them to catalyze highly valuable reactions in organic synthesis. However, the cofactor recycling is often a hurdle to implement enzymes at the industrial level. The fabrication of heterogeneous biocatalysts co‐immobilizing phosphorylated cofactors (PLP, FAD+, and NAD+) and enzymes onto the same solid material is reported to perform chemical reactions without exogeneous addition of cofactors in aqueous media. In these self‐sufficient heterogeneous biocatalysts, the immobilized enzymes are catalytically active and the immobilized cofactors catalytically available and retained into the solid phase for several reaction cycles. Finally, we have applied a NAD+‐dependent heterogeneous biocatalyst to continuous flow asymmetric reduction of prochiral ketones, thus demonstrating the robustness of this approach for large scale biotransformations.
000168150 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BIO2014-61838-EXP$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2015-69887-R
000168150 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttps://creativecommons.org/licenses/by-nc/4.0/deed.es
000168150 590__ $$a12.102$$b2017
000168150 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b14 / 169 = 0.083$$c2017$$dQ1$$eT1
000168150 592__ $$a6.155$$b2017
000168150 593__ $$aChemistry (miscellaneous)$$c2017$$dQ1
000168150 593__ $$aCatalysis$$c2017$$dQ1
000168150 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000168150 700__ $$aBenítez-Mateos, Ana I.
000168150 700__ $$aLópez-Gallego, Fernando
000168150 773__ $$g56, 3 (2017), 771-775$$pAngew. Chem. (Int. ed.)$$tAngewandte Chemie (International ed.)$$x1433-7851
000168150 8564_ $$s1865168$$uhttps://zaguan.unizar.es/record/168150/files/texto_completo.pdf$$yVersión publicada
000168150 8564_ $$s3571292$$uhttps://zaguan.unizar.es/record/168150/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000168150 909CO $$ooai:zaguan.unizar.es:168150$$particulos$$pdriver
000168150 951__ $$a2026-01-27-15:01:22
000168150 980__ $$aARTICLE