000168154 001__ 168154
000168154 005__ 20260127160850.0
000168154 0247_ $$2doi$$a10.1002/cssc.202200397
000168154 0248_ $$2sideral$$a147705
000168154 037__ $$aART-2022-147705
000168154 041__ $$aeng
000168154 100__ $$0(orcid)0000-0001-9478-6750$$aVelasco-Lozano, Susana$$uUniversidad de Zaragoza
000168154 245__ $$aCell‐Free Biosynthesis of ω‐Hydroxy Acids Boosted by a Synergistic Combination of Alcohol Dehydrogenases
000168154 260__ $$c2022
000168154 5060_ $$aAccess copy available to the general public$$fUnrestricted
000168154 5203_ $$aThe activity orchestration of an unprecedented cell-free enzyme system with self-sufficient cofactor recycling enables the stepwise transformation of aliphatic diols into ω-hydroxy acids at the expense of molecular oxygen as electron acceptor. The efficiency of the biosynthetic route was maximized when two compatible alcohol dehydrogenases were selected as specialist biocatalysts for each one of the oxidative steps required for the oxidative lactonization of diols. The cell-free system reached up to 100 % conversion using 100 mM of linear C5 diols and performed the desymmetrization of prochiral branched diols into the corresponding ω-hydroxy acids with an exquisite enantioselectivity (ee>99 %). Green metrics demonstrate superior sustainability of this system compared to traditional metal catalysts and even to whole cells for the synthesis of 5-hydroxypetanoic acid. Finally, the cell-free system was assembled into a consortium of heterogeneous biocatalysts that allowed the enzyme reutilization. This cascade illustrates the potential of systems biocatalysis to access new heterofunctional molecules such as ω-hydroxy acids.
000168154 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/PCI2018-092984$$9info:eu-repo/grantAgreement/ES/MINECO/RTI2018-094398-B-I00
000168154 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000168154 590__ $$a8.4$$b2022
000168154 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b32 / 178 = 0.18$$c2022$$dQ1$$eT1
000168154 591__ $$aGREEN & SUSTAINABLE SCIENCE & TECHNOLOGY$$b14 / 46 = 0.304$$c2022$$dQ2$$eT1
000168154 592__ $$a2.064$$b2022
000168154 593__ $$aChemical Engineering (miscellaneous)$$c2022$$dQ1
000168154 593__ $$aMaterials Science (miscellaneous)$$c2022$$dQ1
000168154 593__ $$aEnvironmental Chemistry$$c2022$$dQ1
000168154 593__ $$aEnergy (miscellaneous)$$c2022$$dQ1
000168154 594__ $$a15.5$$b2022
000168154 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000168154 700__ $$aSantiago-Arcos, Javier
000168154 700__ $$aGrazia Rubanu, Maria
000168154 700__ $$aLópez-Gallego, Fernando
000168154 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000168154 773__ $$g15, 9 (2022)$$pChemSusChem$$tChemSusChem$$x1864-5631
000168154 8564_ $$s2795525$$uhttps://zaguan.unizar.es/record/168154/files/texto_completo.pdf$$yVersión publicada
000168154 8564_ $$s3083202$$uhttps://zaguan.unizar.es/record/168154/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000168154 909CO $$ooai:zaguan.unizar.es:168154$$particulos$$pdriver
000168154 951__ $$a2026-01-27-15:01:28
000168154 980__ $$aARTICLE