000168281 001__ 168281
000168281 005__ 20260202151222.0
000168281 0247_ $$2doi$$a10.1017/s0031182025101066
000168281 0248_ $$2sideral$$a147777
000168281 037__ $$aART-2025-147777
000168281 041__ $$aeng
000168281 100__ $$0(orcid)0000-0001-5750-3643$$aPeris, María Paz$$uUniversidad de Zaragoza
000168281 245__ $$aMolecular survey and risk factors of Trypanosoma pestanai in Eurasian badgers from the northern Iberian Peninsula
000168281 260__ $$c2025
000168281 5060_ $$aAccess copy available to the general public$$fUnrestricted
000168281 5203_ $$aTrypanosoma (Megatrypanum) pestanai is a parasite of the Eurasian badger (Meles meles), reported in various European countries. However, its presence in the Iberian Peninsula had not been previously investigated. To address this knowledge gap and to assess its occurrence and potential risk factors associated with infection, we analyzed DNA from the spleens of 145 badgers sampled across 4 autonomous regions in northern Spain. Two real-time PCR assays using a reference 18S rRNA partial sequence of T. pestanai (92 bp) were developed: one based on SYBR Green chemistry and the other employing a TaqMan probe. Both protocols demonstrated excellent concordance. Defining a sample as positive when at least 1 assay yielded a positive result, the overall prevalence was 35%, consistent with values previously reported in other European populations. A logistic regression model indicated a significantly higher occurrence in badgers from the Eurosiberian bioregion (42%) compared to those from the Mediterranean bioregion (19%). No significant associations were found with age or sex. A subset of positive samples was further analyzed by conventional PCR targeting approximately 900 bp of the 18S rRNA gene and sequenced. All 9 high-quality sequences shared 99.75–100% identity with known T. pestanai sequences. These findings confirm that T. pestanai is a common parasite of Iberian badgers and suggest that more humid climatic conditions may favour its persistence, potentially through effects on host ecology or vector dynamics.
000168281 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A16-23R
000168281 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000168281 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000168281 700__ $$0(orcid)0000-0001-8435-6386$$aRodríguez-Pastor, Ruth
000168281 700__ $$aAstiz, Eva
000168281 700__ $$0(orcid)0000-0002-4121-8087$$aHalaihel, Nabil$$uUniversidad de Zaragoza
000168281 700__ $$aBarral, Marta
000168281 700__ $$aGerrikagoitia, Xeider
000168281 700__ $$aGonzález, Chabier
000168281 700__ $$aUrra, Fermín
000168281 700__ $$aVelarde, Roser
000168281 700__ $$aVillanúa, Diego
000168281 700__ $$aMillán, Javier
000168281 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000168281 773__ $$g(2025), 1-7$$pParasitology$$tPARASITOLOGY$$x0031-1820
000168281 8564_ $$s1982069$$uhttps://zaguan.unizar.es/record/168281/files/texto_completo.pdf$$yVersión publicada
000168281 8564_ $$s3137690$$uhttps://zaguan.unizar.es/record/168281/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000168281 909CO $$ooai:zaguan.unizar.es:168281$$particulos$$pdriver
000168281 951__ $$a2026-02-02-14:39:54
000168281 980__ $$aARTICLE