168287 20260202151222.0 doi 10.1016/j.vprsr.2026.101437 sideral 147776 ART-2026-147776 eng Martínez-Durán, David Universidad de Zaragoza (orcid)0000-0003-2208-1350 Vector-borne pathogens in Spanish greyhounds from Central Spain: Prevalence and hematobiochemical findings 2026 Vector-borne diseases are receiving increasing attention in public health, veterinary medicine, and epidemiological research. In this study, we investigated the seroprevalence of four major canine vector-borne pathogens in Spanish greyhounds, a breed reported to possess distinctive hematological and biochemical profiles. Serum samples from 160 dogs, originating from central Spain, were evaluated for antibodies against Anaplasma phagocytophilum, Ehrlichia canis, and Leishmania infantum using an indirect immunofluorescence assay (IFA), and for circulating Dirofilaria immitis antigens using ELISA. Comprehensive hematological and serum biochemical analyses were also performed. The overall seroprevalence rates were 10% for A. phagocytophilum, 3.1% for E. canis, 13.1% for L. infantum and 0% for D. immitis. A statistically significant association was identified between age and E. canis seropositivity, with higher rates observed in juvenile dogs, whereas L. infantum demonstrated a non-significant trend toward higher prevalence in adults. Seropositive dogs showed notable alterations in hematological and biochemical parameter, including thrombocytopenia, lymphocytosis/lymphopenia, anemia, and variations in platelet indices. These findings highlight the epidemiological relevance of serological screening and detailed clinical monitoring particularly given the breed's frequent involvement in hunting and prolonged exposure to outdoor environments conducive to vector transmission. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Mujika, Maider Morales, Mariano Castillo, Juan Antonio Universidad de Zaragoza (orcid)0000-0002-2048-4749 Moreno, Bernardino Universidad de Zaragoza (orcid)0000-0001-6629-6842 Delacour-Estrella, Sarah Universidad de Zaragoza (orcid)0000-0001-7046-2997 Ruiz-Arrondo, Ignacio Universidad de Zaragoza (orcid)0000-0001-8198-8118 Peris, María Paz Universidad de Zaragoza (orcid)0000-0001-5750-3643 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 68 (2026), 101437 [ 6 pp.] Veterinary Parasitology: Regional Studies and Reports 2405-9390 488639 http://zaguan.unizar.es/record/168287/files/texto_completo.pdf Versión publicada 2743596 http://zaguan.unizar.es/record/168287/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:168287 articulos driver 2026-02-02-14:40:01 ARTICLE