000168379 001__ 168379
000168379 005__ 20260204153543.0
000168379 0247_ $$2doi$$a10.3390/vetsci13020130
000168379 0248_ $$2sideral$$a147799
000168379 037__ $$aART-2026-147799
000168379 041__ $$aeng
000168379 100__ $$0(orcid)0000-0002-2497-9028$$aBenito, Alfredo A.
000168379 245__ $$aFirst Detection and Genetic Characterization of Influenza D Virus in Cattle in Spain
000168379 260__ $$c2026
000168379 5060_ $$aAccess copy available to the general public$$fUnrestricted
000168379 5203_ $$aInfluenza D virus (IDV) and co-circulating respiratory pathogens were investigated using RT-qPCR in 316 samples collected from 210 Spanish cattle farms with bovine respiratory disease (BRD) outbreaks between July 2023 and September 2024. Thirty-eight IDV-positive samples, from 30 farms across 15 provinces throughout Spain, were identified. IDV was significantly more frequent in bronchoalveolar lavage samples (22.1%) and nasal swabs (13.5%) compared to lung tissues (5%) and other sample mixtures (5%). All IDV-positive specimens exhibited co-infections, with most (94.7%) harboring three to seven viral and/or bacterial pathogens, highlighting the complexity of BRD outbreaks. Cramer’s V analysis revealed moderate IDV association with Mycoplasma bovis (V = 0.255) and Pasteurella multocida (V = 0.223), and a weaker IDV-bovine coronavirus association (V = 0.202), while IDV association with Histophilus somni (V = 0.025) and bovine herpesvirus 1 (V = 0.000) was negligible. Partial sequences of the hemagglutinin-esterase (HEF) gene from a subset of 13 IDV-positive samples (Ct < 32) were obtained. This confirmed the presence of the two major genetic lineages detected among cattle in Europe, D/OK and D/660, with D/660 exhibiting higher genetic diversity, as determined by DNAsp 6.12 software. This is the first report of IDV infection in Spanish cattle, confirming the circulation of the D/OK and D/660 lineages within the cattle population.
000168379 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000168379 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000168379 700__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, Luis V.$$uUniversidad de Zaragoza
000168379 700__ $$aLázaro-Gaspar, Sofía
000168379 700__ $$0(orcid)0000-0001-7106-0068$$aGarza-Moreno, Laura$$uUniversidad de Zaragoza
000168379 700__ $$aAntón-Baltanás, Nuria
000168379 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, Joaquín$$uUniversidad de Zaragoza
000168379 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000168379 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000168379 773__ $$g13, 2 (2026), 130 [11 pp.]$$tVeterinary Sciences$$x2306-7381
000168379 8564_ $$s800596$$uhttps://zaguan.unizar.es/record/168379/files/texto_completo.pdf$$yVersión publicada
000168379 8564_ $$s2502169$$uhttps://zaguan.unizar.es/record/168379/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000168379 909CO $$ooai:zaguan.unizar.es:168379$$particulos$$pdriver
000168379 951__ $$a2026-02-04-13:14:51
000168379 980__ $$aARTICLE