000168444 001__ 168444
000168444 005__ 20260205155159.0
000168444 0247_ $$2doi$$a10.1007/s13105-026-01151-9
000168444 0248_ $$2sideral$$a147880
000168444 037__ $$aART-2026-147880
000168444 041__ $$aeng
000168444 100__ $$aMillán-Laleona, Adrián
000168444 245__ $$aProcyanidin B2 and an autochthonous apple pulp extract modulate oxidative stress and PPARγ expression on an in vitro model of lipid steatosis in HepG2 cells
000168444 260__ $$c2026
000168444 5060_ $$aAccess copy available to the general public$$fUnrestricted
000168444 5203_ $$aMetabolic dysfunction-associated steatotic liver disease (MASLD) is characterized by hepatic steatosis alongside metabolic comorbidities like type 2 diabetes mellitus (T2DM), dyslipidaemia, overweight, or obesity. Liver damage could be prevented promoting consumption of fruits and vegetables, the main source of phenolic compounds. These molecules have been previously related with antioxidant and anti-obesity properties. Therefore, the objective of this study is to determine the antioxidant capacity and potential preventive activity of an autochthonous apple pulp extract, Amarilla de Octubre, and its main phenolic compound, procyanidin B2 (PB2), in an in vitro model of liver steatosis. Antioxidant activity was assessed by xanthine/xanthine oxidase (X/XO) system and nitric oxide scavenging production. Monoamine oxidase A (MAO-A) inhibition was also determined due to its implication in detoxification processes in liver. Human hepatocytes (HepG2 cell line) were selected to test their potential cytotoxicity, oxidative stress situations (ROS production) and the potential effect in lipid metabolism by overloading cells with oleic acid. Fat accumulation and gene expression related to lipid metabolism (PPARγ, CD36 and FAS) were also analysed by Oil Red O and qPCR respectively. PB2 showed promising results as an antioxidant compound in scavenging and enzymatic inhibition-related experiments. However, Amarilla de Octubre not only reduce fat accumulation, but also modulates the expression of PPARγ, CD36, and FAS and demonstrates slightly lower efficacy than PB2 in scavenging and enzymatic inhibition-related experiments. These results highlight the potential of apples to mitigate pathogenic conditions associated with metabolic disorders such as obesity, emphasizing the role of dietary polyphenols in this protective effect.
000168444 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B44-23R$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PID2019-108081RR-C21$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PID2022-141847OR-C33$$9info:eu-repo/grantAgreement/ES/MINECO/PID2022-141313OB-I00
000168444 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000168444 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000168444 700__ $$0(orcid)0000-0002-5822-9823$$aLopez-Yus, Marta
000168444 700__ $$0(orcid)0000-0002-1033-6152$$aLorente-Cebrián, Silvia$$uUniversidad de Zaragoza
000168444 700__ $$0(orcid)0000-0002-8982-3737$$aArbones-Mainar, Jose M.
000168444 700__ $$aGómez-Rincón, Carlota
000168444 700__ $$0(orcid)0000-0001-6969-1055$$aLópez, Víctor
000168444 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000168444 773__ $$g82, 4 (2026), [12 pp.]$$pJ. physiol. biochem.$$tJournal of Physiology and Biochemistry$$x1138-7548
000168444 8564_ $$s2365221$$uhttps://zaguan.unizar.es/record/168444/files/texto_completo.pdf$$yVersión publicada
000168444 8564_ $$s2047450$$uhttps://zaguan.unizar.es/record/168444/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000168444 909CO $$ooai:zaguan.unizar.es:168444$$particulos$$pdriver
000168444 951__ $$a2026-02-05-14:36:43
000168444 980__ $$aARTICLE