168457 20260205155159.0 doi 10.1016/j.foodcont.2025.111930 sideral 147913 ART-2025-147913 eng Esteban-Sanz, Clara Universidad de Zaragoza (orcid)0009-0005-0336-7582 ELISA and lateral flow immunoassay validation for a sensitive and rapid detection of crustaceans in thermally processed food and working surfaces 2025 Crustaceans are one of the major triggers of food allergies and can be found as hidden allergens. A sandwich ELISA and lateral flow immunoassay (LFIA) were developed and validated to detect crustaceans by identifying prawn tropomyosin. Both methods could detect crustacean protein in complex food matrices spiked with prawn extract at levels of 1.25 and 5 μg/g, respectively, with high recoveries (59 %–98 %). Crustacean protein was also identified in model thermally processed foods (sausages, croquettes, and broth) containing prawn at levels of 1.25 and 10 μg/g using ELISA and LFIA. Among 63 basic ingredients analyzed, cross-reactivity was observed with mollusks, enabling their detection. ELISA and LFIA could detect 0.1 and 1.0 μg of crustacean protein on contact surfaces, respectively. Both tests proved to be robust. Laboratory and on-site analysis using these immunoassays can support allergen management strategies, helping to prevent unnecessary precautionary labelling. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/A20-23R info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Civera, Alba (orcid)0000-0001-7719-6388 Mata, Luis Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X Pérez, María D. Galan-Malo, Patricia Universidad de Zaragoza 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 183 (2025), 111930 [11 pp.] Food control Food Control 0956-7135 859652 http://zaguan.unizar.es/record/168457/files/texto_completo.pdf Versión publicada 2572430 http://zaguan.unizar.es/record/168457/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:168457 articulos driver 2026-02-05-14:36:58 ARTICLE