000168468 001__ 168468 000168468 005__ 20260217205613.0 000168468 0247_ $$2doi$$a10.1039/d4tb01503g 000168468 0248_ $$2sideral$$a147897 000168468 037__ $$aART-2024-147897 000168468 041__ $$aeng 000168468 100__ $$aKaur, Impreet 000168468 245__ $$aIn vivo transplantation of intrahepatic cholangiocyte organoids with decellularized liver-derived hydrogels supports hepatic cellular proliferation and differentiation in chronic liver injury 000168468 260__ $$c2024 000168468 5060_ $$aAccess copy available to the general public$$fUnrestricted 000168468 5203_ $$aThe limited replicative potential of primary hepatocytes (Hep) is a major hurdle for obtaining sufficient quantity and quality hepatocytes during cell therapy in patients with liver failure. Intrahepatic cholangiocyte organoids (ICOs) derived from intrahepatic bile ducts differentiate into both hepatocytes and cholangiocytes in vitro. Here, we studied in vivo effects of transplanting ICOs and Hep in chronic liver injury mice models. Well characterized primary mouse ICOs and Hep were mixed in decellularized liver (DCL) matrix hydrogels and injected into the subcapsular left lateral liver lobe of CCl4-induced liver injury models whereas mice given DCL alone were in the sham group. Two weeks post-transplantation, transplanted liver lobes were collected and studied by histology and RNA sequencing. Transplanted animals did not exhibit any tumors, mortality or morbidity. Mice livers transplanted with ICOs had increased cellular proliferation and vascularization as compared to Hep transplanted mice or sham. Collagen deposition in the liver was significantly reduced and serum albumin levels were significantly increased in transplanted groups compared to the sham group. Expression of genes associated with hepatocyte differentiation was highest in Hep transplanted livers among the three groups, but they were also upregulated in ICO transplanted livers compared to sham. Our study demonstrates that ICOs encapsulated in DCL hydrogels when transplanted in chronically injured mice livers engraft well and show hepatocyte differentiation and reduction of fibrosis, indicating that hydrogel transplanted cholangiocyte organoids may serve as an efficient cell source and therapy for renewal of hepatocytes, restoration of hepatocyte functions and resolution of liver injury. 000168468 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000168468 590__ $$a5.8$$b2024 000168468 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b16 / 55 = 0.291$$c2024$$dQ2$$eT1 000168468 592__ $$a1.159$$b2024 000168468 593__ $$aBiomedical Engineering$$c2024$$dQ1 000168468 593__ $$aMedicine (miscellaneous)$$c2024$$dQ1 000168468 593__ $$aMaterials Science (miscellaneous)$$c2024$$dQ1 000168468 593__ $$aChemistry (miscellaneous)$$c2024$$dQ1 000168468 594__ $$a10.4$$b2024 000168468 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000168468 700__ $$aVasudevan, Ashwini 000168468 700__ $$aSanchez-Romero, Natalia 000168468 700__ $$aSanyal, Arka 000168468 700__ $$aSharma, Aarushi 000168468 700__ $$aHemati, Hamed 000168468 700__ $$aJuneja, Pinky 000168468 700__ $$aSharma, Aarti 000168468 700__ $$aPla Palacin, Iris 000168468 700__ $$aRastogi, Archana 000168468 700__ $$aVijayaragavan, Pooja 000168468 700__ $$aGhosh, Sourabh 000168468 700__ $$aRamakrishna, Seeram 000168468 700__ $$aSarin, Shiv K. 000168468 700__ $$aBaptista, Pedro M. 000168468 700__ $$aTripathi, Dinesh M. 000168468 700__ $$aKaur, Savneet 000168468 773__ $$g13, 3 (2024), 918-928$$pJ. mater. chem. B$$tJournal of Materials Chemistry B$$x2050-750X 000168468 8564_ $$s1816556$$uhttps://zaguan.unizar.es/record/168468/files/texto_completo.pdf$$yPostprint 000168468 8564_ $$s1140798$$uhttps://zaguan.unizar.es/record/168468/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000168468 909CO $$ooai:zaguan.unizar.es:168468$$particulos$$pdriver 000168468 951__ $$a2026-02-17-20:49:33 000168468 980__ $$aARTICLE