000168483 001__ 168483
000168483 005__ 20260209162329.0
000168483 0248_ $$2sideral$$a64934
000168483 037__ $$aART-2009-64934
000168483 041__ $$aeng
000168483 100__ $$0(orcid)0000-0003-4010-849X$$aGoñi, G
000168483 245__ $$aCyanobacterial Electron Carrier Proteins as Electron Donors to CYP106A2 from Bacillus Megaterium ATCC 13368
000168483 260__ $$c2009
000168483 5060_ $$aAccess copy available to the general public$$fUnrestricted
000168483 5203_ $$aThe CYP450 from Bacillus megaterium (BmCYP106A2) catalyzes the 15βhydroxylation of several steroids and also synthesizes mono-hydroxylated 9α- and 11αOH-progesterone BmCYP106A2. The present study reports on the ability of BmCYP106A2 to be efficiently reduced by a truncated bovine adrenodoxin mutant, as well as by the photosynthetic flavodoxin and particularly ferredoxin electron carriers from the cyanobacterium Anabaena. These results open the possibility for the design of a hybrid system to provide reducing equivalents for the hydroxylation process. Additionally, they suggest that despite the interaction of BmCYP106A2 with these
proteins, particularly with flavodoxin, do not rely on a precise complementarity of the reacting molecules, rearrangements might be required and alternative binding modes might contribute to the observed ET reactions.
000168483 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000168483 590__ $$a2.48$$b2009
000168483 591__ $$aBIOPHYSICS$$b41 / 74 = 0.554$$c2009$$dQ3$$eT2
000168483 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b153 / 281 = 0.544$$c2009$$dQ3$$eT2
000168483 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000168483 700__ $$aZoellner, A
000168483 700__ $$aLisurek, M
000168483 700__ $$0(orcid)0000-0001-5702-4538$$aVelazquez-Campoy, A$$uUniversidad de Zaragoza
000168483 700__ $$aPinto, S
000168483 700__ $$0(orcid)0000-0003-4087-4733$$aGomez-Moreno, C$$uUniversidad de Zaragoza
000168483 700__ $$aHannemann, F
000168483 700__ $$aBernhardt, R
000168483 700__ $$0(orcid)0000-0001-8743-0182$$aMedina, M.$$uUniversidad de Zaragoza
000168483 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000168483 773__ $$g1794, 11 (2009), 1635-1642$$pBBA-Proteins Proteomics$$tBiochimica et Biophysica Acta - Proteins and Proteomics$$x1570-9639
000168483 8564_ $$s347383$$uhttps://zaguan.unizar.es/record/168483/files/texto_completo.pdf$$yPostprint
000168483 8564_ $$s1056169$$uhttps://zaguan.unizar.es/record/168483/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000168483 909CO $$ooai:zaguan.unizar.es:168483$$particulos$$pdriver
000168483 951__ $$a2026-02-09-14:41:49
000168483 980__ $$aARTICLE