168483 20260209162329.0 sideral 64934 ART-2009-64934 eng Goñi, G (orcid)0000-0003-4010-849X Cyanobacterial Electron Carrier Proteins as Electron Donors to CYP106A2 from Bacillus Megaterium ATCC 13368 2009 The CYP450 from Bacillus megaterium (BmCYP106A2) catalyzes the 15βhydroxylation of several steroids and also synthesizes mono-hydroxylated 9α- and 11αOH-progesterone BmCYP106A2. The present study reports on the ability of BmCYP106A2 to be efficiently reduced by a truncated bovine adrenodoxin mutant, as well as by the photosynthetic flavodoxin and particularly ferredoxin electron carriers from the cyanobacterium Anabaena. These results open the possibility for the design of a hybrid system to provide reducing equivalents for the hydroxylation process. Additionally, they suggest that despite the interaction of BmCYP106A2 with these proteins, particularly with flavodoxin, do not rely on a precise complementarity of the reacting molecules, rearrangements might be required and alternative binding modes might contribute to the observed ET reactions. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 2.48 2009 BIOPHYSICS 41 / 74 = 0.554 2009 Q3 T2 BIOCHEMISTRY & MOLECULAR BIOLOGY 153 / 281 = 0.544 2009 Q3 T2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Zoellner, A Lisurek, M Velazquez-Campoy, A Universidad de Zaragoza (orcid)0000-0001-5702-4538 Pinto, S Gomez-Moreno, C Universidad de Zaragoza (orcid)0000-0003-4087-4733 Hannemann, F Bernhardt, R Medina, M. Universidad de Zaragoza (orcid)0000-0001-8743-0182 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1794, 11 (2009), 1635-1642 BBA-Proteins Proteomics Biochimica et Biophysica Acta - Proteins and Proteomics 1570-9639 347383 http://zaguan.unizar.es/record/168483/files/texto_completo.pdf Postprint 1056169 http://zaguan.unizar.es/record/168483/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:168483 articulos driver 2026-02-09-14:41:49 ARTICLE