168496 20260209162330.0 doi 10.1089/vbz.2020.2662 sideral 137587 ART-2020-137587 eng Ruiz-Arrondo, I. Universidad de Zaragoza (orcid)0000-0001-8198-8118 Integrated Approaches in Support of Taxonomic Identification of Mosquitoes (Diptera: Culicidae) in Vector Surveillance in Spain 2020 The pandemic of Zika virus in 2016 and other arboviruses prompted La Rioja Government in Spain to implement an entomological surveillance program of mosquitoes (Diptera; Culicidae) in the region of La Rioja. The morphological identification was supported by genetic analysis using the COI (cytochrome c oxidase subunit I) and the ITS2 (internal transcribed spacer 2) genes. In total, we identified 24 species arranged in 6 genera: Aedes (7 species), Anopheles (4 species), Coquillettidia (1 species), Culex (7 species), Culiseta (4 species), and Uranotaenia (1 species). Aedes sticticus and Aedes geniculatus are newly reported for La Rioja region. In total, 465 COI sequences were analyzed for Culicinae and Anophelinae and 54 ITS2 sequences for Anophelinae; all individuals identified as the same species clustered together in the Neighbor Joining trees. The levels of sequence divergence based on COI ranged between 0% and 2.62%, while the interspecific genetic divergence ranged from 3.05% to 20.07%. Within the genus Culiseta, certain specimens of Culiseta annulata, Culiseta litorea, and Culiseta subochrea were morphologically misidentified due to variation in the main diagnostic characters. The interspecific genetic divergence based on the ITS2 ranged from 0% to 2.98%. An accurate identification of mosquito vectors is the first step to establish a vector surveillance program for preventing pathogen transmission. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.133 2020 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH 137 / 202 = 0.678 2020 Q3 T3 INFECTIOUS DISEASES 77 / 92 = 0.837 2020 Q4 T3 0.838 2020 Infectious Diseases 2020 Q2 Virology 2020 Q2 Microbiology 2020 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Hernández-Triana, L.M. Nikolova, N.I. Fooks, A.R. Oteo, J.A. 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 20, 11 (2020), 831-842 Vector-Borne Zoonotic Dis. VECTOR-BORNE AND ZOONOTIC DISEASES 1530-3667 881858 http://zaguan.unizar.es/record/168496/files/texto_completo.pdf Versión publicada 2622820 http://zaguan.unizar.es/record/168496/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:168496 articulos driver 2026-02-09-14:42:10 ARTICLE