168554 20260211123807.0 doi 10.1016/j.jbiomech.2015.10.026 sideral 94948 ART-2016-94948 eng Del Amo, C. Universidad de Zaragoza (orcid)0000-0002-3796-4482 Quantification of angiogenic sprouting under different growth factors in a microfluidic platform 2016 Angiogenesis, as example of collective migration of endothelial cells (ECs), is the main dynamic process that culminates in sprout formation from existing vessels. After tissue injury, the vascularity is interrupted, triggering the regeneration process and the release of different growth factors (GFs). The main aim of this work is to quantify the effect of specific GFs during the initial stage of sprout formation, namely: VEGF, PDGF-BB, TGFß and BMP-2, all of them involved in regenerative processes. For this purpose, we designed a novel algorithm implemented in Matlab to quantify the advance of the EC monolayer over time and the sprout structure in 3D. Our results show that VEGF is the main regulatory GF on angiogenesis processes, producing longer sprouts with higher frequency. However, the chemoattractant effect of VEGF depends on its concentration and its spatiotemporal location, having a critical impact on the sprout time evolution. PDGF-BB (namely as PDGF) has a global negative effect on both the number and length of sprouts. TGFß enhances sprout length at earlier times, although its effect gradually disappears over time. Finally, BMP-2 produces, overall, less number and shorter sprouts, but was the only GF with a positive evolution at longer times, producing fewer but longer sprouts. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751 info:eu-repo/grantAgreement/ES/MEC/FPU13-03194 info:eu-repo/grantAgreement/ES/MINECO/DPI2012-38090-C03-01 info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 2.664 2016 ENGINEERING, BIOMEDICAL 25 / 77 = 0.325 2016 Q2 T1 BIOPHYSICS 32 / 73 = 0.438 2016 Q2 T2 1.242 2016 Biomedical Engineering 2016 Q1 Biophysics 2016 Q1 Sports Science 2016 Q1 Rehabilitation 2016 Q1 Orthopedics and Sports Medicine 2016 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Borau, C. Universidad de Zaragoza (orcid)0000-0002-3784-1140 Gutiérrez, R. Asín, J. Universidad de Zaragoza (orcid)0000-0002-0174-789X García-Aznar, J.M. Universidad de Zaragoza (orcid)0000-0002-9864-7683 2007 265 Universidad de Zaragoza Dpto. Métodos Estadísticos Área Estadís. Investig. Opera. 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 49, 8 (2016), 1340-1346 J. biomech. Journal of Biomechanics 0021-9290 1118432 http://zaguan.unizar.es/record/168554/files/texto_completo.pdf Postprint 2823363 http://zaguan.unizar.es/record/168554/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:168554 articulos driver 2026-02-11-10:27:03 ARTICLE