000168599 001__ 168599
000168599 005__ 20260211123813.0
000168599 0247_ $$2doi$$a10.1080/1828051X.2026.2622223
000168599 0248_ $$2sideral$$a148006
000168599 037__ $$aART-2026-148006
000168599 041__ $$aeng
000168599 100__ $$0(orcid)0009-0005-9117-8617$$aMuñoz-Grein, Jennifer$$uUniversidad de Zaragoza
000168599 245__ $$aIn vitro effects of saponins, tannins and lipids on rumen fermentation and intestinal health markers in a ruminant weaning context
000168599 260__ $$c2026
000168599 5060_ $$aAccess copy available to the general public$$fUnrestricted
000168599 5203_ $$aDairy ruminants often experience physiological disturbances during the post-weaning, includingsuboptimal rumen fermentation due to an underdeveloped rumen microbiota, and diarrhoeaassociated with intestinal inflammation and oxidative stress. This study explored six commercialfeed additives with a potential dual mode of action to optimise both rumen fermentation andintestinal health in a post-weaning scenario. The experimental treatments included dietary sup-plementation with saponins (SAP), tannins (TAN), lauric acid (LAU), fish oil (FIO), high-unsaturatedolein (HUO) and high-saturated olein (HSO). Additives were tested at four doses (0, 100, 300 and600 mg/L) with four experimental replicates. Effects on rumen fermentation were evaluated inbatch cultures using inocula from dairy lambs and a high concentrate substrate, while intestinalhealth responses were evaluated in Caco-2 cells. Saponins (p ¼ 0.01) and tannins (p < 0.01) lin-early decreased rumen ammonia-N concentration in batch cultures. In Caco-2 cells, saponinsimproved metabolic activity (p ¼ 0.04), whereas tannins reduced pro-inflammatory IL-6 concentra-tion (p < 0.01), suggesting beneficial effects on intestinal health. Lipid sources exerted minimaleffects on rumen fermentation and induced intestinal inflammation when supplemented at highdoses. Overall, the physiological constrains of dairy ruminants during the post-weaning period,such as an immature rumen microbiota, absence of protozoa and low rumen pH, may limit theefficacy of additives in improving rumen fermentation. Nevertheless, saponins and tanninsshowed potential to reduce rumen proteolysis and support intestinal health during this criticaldevelopmental stage. Further in vivo research is required to validate these findings.
000168599 536__ $$9info:eu-repo/grantAgreement/ES/AEI/MICNN/PID2021-123206OB-I00$$9info:eu-repo/grantAgreement/ES/MICINN PRE2022-101806$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2019-027764-I/AEI/10.13039/501100011033
000168599 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000168599 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000168599 700__ $$0(orcid)0000-0001-8056-5236$$aSolanas, Estela$$uUniversidad de Zaragoza
000168599 700__ $$0(orcid)0000-0002-0712-1185$$aFondevila, Manuel$$uUniversidad de Zaragoza
000168599 700__ $$0(orcid)0000-0001-5880-6021$$aBelanche, Alejandro$$uUniversidad de Zaragoza
000168599 7102_ $$11003$$2027$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Anatom.Embriol.Humana
000168599 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000168599 773__ $$g25, 1 (2026), 125-137$$pItal. J. Anim. Sci.$$tItalian Journal of Animal Science$$x1594-4077
000168599 8564_ $$s1410883$$uhttps://zaguan.unizar.es/record/168599/files/texto_completo.pdf$$yVersión publicada
000168599 8564_ $$s1065623$$uhttps://zaguan.unizar.es/record/168599/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000168599 909CO $$ooai:zaguan.unizar.es:168599$$particulos$$pdriver
000168599 951__ $$a2026-02-11-10:28:00
000168599 980__ $$aARTICLE